Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (38)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Krausz, C.
Right arrow Articles by Baldi, E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Krausz, C.
Right arrow Articles by Baldi, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 10, No. 1, pp. 120-124, 1995
© 1995 European Society of Human Reproduction and Embryology

Intracellular calcium increase and acrosome reaction in response to progesterone in human spermatozoa are correlated with in-vitro fertilization

Csilla Krausz, Lorella Bonaccorsi, Michaela Luconi, Beatrice Fuzzi1, Luciana Criscuoli1, Sandra Pellegrini1, Gianni Forti and Elisabetta Baldi2

Dipartimento di Fisiopatologia Clinica, Unita di Andrologidà and Università di Firenze viale Piericcini, 6-50139 Firenze, Italy 1 Clinica Ostetrica e Ginecologica, Università di Firenze viale Piericcini, 6-50139 Firenze, Italy

Correspondence: 2To whom correspondence should be addressed

In this study we have investigated responsiveness to progesterone in spermatozoa from a group of unselected male partners of couples undergoing in-vitro fertilization (IVF). We evaluated progesterone-stimulated intracellular Ca2+ ([Ca2+]i) and percentage increase in acrosome reaction in the same sperm sample used for oocyte inseminations. [Ca2+]i was measured with a fluorimetric method, while the acrosome reaction was assessed using a fluorescent probe (fluorescein isothiocyanate-labelled peanut lectin). The average percentage [Ca2+]i as well as the rate of increase in the frequency of acrosome reaction following progesterone challenge were significantly lower (P < 0.005) in the group of patients with a fertilization rate <50%. In addition, significant correlations between the fertilization rate and the progesterone-stimulated [Ca2+]i and acrosome reaction increases (r = 0.78 and r = 0.79 respectively) were observed. Furthermore, in cases of fertilization failure, no increase of [Ca2+]i or acrosome reaction was observed in response to progesterone with the exception of one case. Our results indicate that [Ca2+]i and acrosome reaction increases in response to progesterone can be of value in the prediction of sperm fertilizing ability. As the two parameters were significantly correlated to each other (r = 0.86), the two assays have similar IVF predictive value and might be used interchangeably as a diagnostic tool in the assignment of male patients to the different kinds of assisted fertilization techniques.

Key words: acrosome reaction/intracellular calcium/in-vitro fertilization/progesterone

Submitted on April 21, 1994; accepted on September 12, 1994.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
S. Costello, F. Michelangeli, K. Nash, L. Lefievre, J. Morris, G. Machado-Oliveira, C. Barratt, J. Kirkman-Brown, and S. Publicover
Ca2+-stores in sperm: their identities and functions
Reproduction, September 1, 2009; 138(3): 425 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. F. Malo, J. J. Garde, A. J. Soler, A. J. Garcia, M. Gomendio, and E. R.S. Roldan
Male Fertility in Natural Populations of Red Deer Is Determined by Sperm Velocity and the Proportion of Normal Spermatozoa
Biol Reprod, April 1, 2005; 72(4): 822 - 829.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Y. Liu and H.W.G. Baker
High frequency of defective sperm-zona pellucida interaction in oligozoospermic infertile men
Hum. Reprod., February 1, 2004; 19(2): 228 - 233.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
J. C. Kirkman-Brown, E. L. Punt, C. L. R. Barratt, and S. J. Publicover
Zona Pellucida and Progesterone-Induced Ca2+ Signaling and Acrosome Reaction in Human Spermatozoa
J Androl, May 1, 2002; 23(3): 306 - 315.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Jagannathan, E. L. Punt, Y. Gu, C. Arnoult, D. Sakkas, C. L. R. Barratt, and S. J. Publicover
Identification and Localization of T-type Voltage-operated Calcium Channel Subunits in Human Male Germ Cells. EXPRESSION OF MULTIPLE ISOFORMS
J. Biol. Chem., March 1, 2002; 277(10): 8449 - 8456.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
B. Rosenbusch, M. Schneider, R. Kreienberg, and C. Brucker
Cytogenetic analysis of human zygotes displaying three pronuclei and one polar body after intracytoplasmic sperm injection
Hum. Reprod., November 1, 2001; 16(11): 2362 - 2367.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Moomjy, L. T. Colombero, L. L. Veeck, Z. Rosenwaks, and G. D. Palermo
Sperm integrity is critical for normal mitotic division and early embryonic development
Mol. Hum. Reprod., September 1, 1999; 5(9): 836 - 844.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Luconi, L. Bonaccorsi, M. Maggi, P. Pecchioli, C. Krausz, G. Forti, and E. Baldi
Identification and Characterization of Functional Nongenomic Progesterone Receptors on Human Sperm Membrane
J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 877 - 885.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.