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Human Reproduction, Vol. 10, No. 1, pp. 132-136, 1995
© 1995 European Society of Human Reproduction and Embryology

Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection

K.M. Sultan, S. Munné1, G.D. Palermo, M. Alikani and J. Cohen

Cornell University Medical College New York, NY 10021, USA

Correspondence: 1To whom correspondence should be addressed at: The Gamete and Embryo Research Laboratory, Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital, Cornell University Medical Center, PO Box 30, 1300 York Avenue, New York, NY 10021, USA

Uni-pronuclear embryos (n = 42) were analysed by fluorescence in-situ hybridization (FISH) with two to four chromosome pair-specific probes. Half of these embryos resulted from conventional insemination and half from intracytoplasmic sperm injection (ICSI). The majority of uni-pronuclear embryos from conventional insemination were normally diploid (61.9%) whereas only 9.5% of uni-pronuclear ICSI embryos (P < 0.001) were diploid. In addition, a significantly higher number of uni-pronuclear embryos from conventional insemination had a Y chromosome (10/21, 47.6%) when compared with ICSI embryos (2/21, 9.5%) (P = 0.015). It is concluded that the majority of uni-pronuclear embryos following regular in-vitro fertilization are fertilized, whereas those from ICSI are parthenogenetically activated. The latter embryos should not be considered for embryo replacement.

Key words: activation/fluorescence in-situ hybridization/ICSI/in-vitro fertilization

Submitted on April 28, 1994; accepted on July 27, 1994.


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