Human Reproduction, Vol. 10, No. 1, pp. 19-23, 1995
© 1995 European Society of Human Reproduction and Embryology
Identification of nitric oxide synthase in human uterus
Department of Obstetrics and Gynaecology, University of Glasgow 10 Alexandra Parade, Glasgow G31 2ER, UK
Correspondence: 1To whom correspondence should be addressed at: The Queen Mother's Hospital, Yorkhill, Glasgow G3 8SJ, UK
The aim of this study was to investigate the presence of nitric oxide synthase (NOS) in human uterus. Tissues were obtained at operation from 10 women undergoing hysterectomy for benign disease. In-situ hybridization was used to determine the distribution of mRNA for NOS with a 483 bp digoxigenin-labelled antisense riboprobe. Localization of NOS was detected by (i) immunocytochemistry using a monoclonal antibody raised against bovine constitutive endothelial NOS, and (ii) NADPH diaphorase, which has been suggested to co-localize with brain NOS. Messenger RNA for NOS was detected in endometrium and myometrium from nine of 10 women, predominantly in endometrial glandular epithelium and stroma and myometrial blood vessels. NOS-like immunoreactivity was seen in endometrial stroma and myometrial blood vessels, whereas NADPH diaphorase activity was localized mainly to endometrial glandular epithelium and myometrial blood vessels. These studies suggest that different forms of constitutive NOS are present in human endometrium and myometrium, and that nitric oxide may play a role in the paracrine control of the uterine vascular bed.
Key words: endometrium/myometrium/nitric oxide/uterus
Submitted on June 1, 1994; accepted on September 20, 1994.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Zicari, C. Centonze, M. Realacci, B. Buchetti, A. Pietropolli, and C. Ticconi Estradiol 17-{beta} and Progesterone Modulate Inducible Nitric Oxide Synthase and High Mobility Group Box 1 Expression in Human Endometrium Reproductive Sciences, July 1, 2008; 15(6): 559 - 566. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Sengupta, L. Dhawan, P G L Lalitkumar, and D Ghosh Nitric oxide in blastocyst implantation in the rhesus monkey Reproduction, September 1, 2005; 130(3): 321 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.H. Shaamash and M.M. Zakhari Increased serum levels of nitric oxide metabolites among users: a possible role in progestin-induced bleeding Hum. Reprod., January 1, 2005; 20(1): 302 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Nath, J. Enciso, M. Kuniyasu, X.-Y. Hao, J. A. Madri, and E. Pinter Nitric oxide modulates murine yolk sac vasculogenesis and rescues glucose induced vasculopathy Development, May 15, 2004; 131(10): 2485 - 2496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Piccinini, R. A. Fano, A. Volpe, and F. Facchinetti Ripening of the Cervix With Sodium Nitroprusside in Nonpregnant Women Reproductive Sciences, October 1, 2003; 10(7): 438 - 442. [Abstract] [PDF]
|
|
|
|
|
|
P. Hoffmann, F. Stanke-Labesque, R. Fanchin, N. Dilai, J.C. Pons, and J.M. Ayoubi Effects of L-arginine and sodium nitroprusside on the spontaneous contractility of human non-pregnant uterus Hum. Reprod., January 1, 2003; 18(1): 148 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Telfer, H. Itoh, A. J. Thomson, J. E. Norman, K. Nakao, J. S. Campa, L. Poston, R. M. Tribe, and R. R. Magness Activity and Expression of Soluble and Particulate Guanylate Cyclases in Myometrium from Nonpregnant and Pregnant Women: Down-Regulation of Soluble Guanylate Cyclase at Term J. Clin. Endocrinol. Metab., December 1, 2001; 86(12): 5934 - 5943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-A. Ledingham, A. J. Thomson, A. Young, L. M. Macara, I. A. Greer, and J. E. Norman Changes in the expression of nitric oxide synthase in the human uterine cervix during pregnancy and parturition Mol. Hum. Reprod., November 1, 2000; 6(11): 1041 - 1048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Naghashpour and G. Dahl Relaxation of Myometrium by Calcitonin Gene-Related Peptide Is Independent of Nitric Oxide Synthase Activity in Mouse Uterus Biol Reprod, November 1, 2000; 63(5): 1421 - 1427. [Abstract] [Full Text]
|
|
|
|
|
|
G. M. Tiboni and F. Giampietro Inhibition of nitric oxide synthesis causes preterm delivery in the mouse Hum. Reprod., August 1, 2000; 15(8): 1838 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Francavilla, R. Santucci, B. Macerola, G. Ruvolo, and R. Romano Nitric Oxide Synthase Inhibition in Human Sperm Affects Sperm-Oocyte Fusion but Not Zona Pellucida Binding Biol Reprod, August 1, 2000; 63(2): 425 - 429. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Gawronska, G. Bodek, and A. J. Ziecik Distribution of NADPH-diaphorase and Nitric Oxide Synthase (NOS) in Different Regions of Porcine Oviduct During the Estrous Cycle J. Histochem. Cytochem., June 1, 2000; 48(6): 867 - 876. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Skarzynski, Y. Miyamoto, and K. Okuda Production of Prostaglandin F2{alpha} by Cultured Bovine Endometrial Cells in Response to Tumor Necrosis Factor {alpha}: Cell Type Specificity and Intracellular Mechanisms Biol Reprod, May 1, 2000; 62(5): 1116 - 1120. [Abstract] [Full Text]
|
|
|
|
|
|
N. Yoshiki, T. Kubota, and T. Aso Expression and localization of inducible nitric oxide synthase in human non-pregnant and early pregnant endometrium Mol. Hum. Reprod., March 1, 2000; 6(3): 283 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Taguchi, J. Alfer, K. Chwalisz, H.M. Beier, and I. Classen-Linke Endothelial nitric oxide synthase is differently expressed in human endometrial vessels during the menstrual cycle Mol. Hum. Reprod., February 1, 2000; 6(2): 185 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zervou, L.D. Klentzeris, and R.W. Old Nitric oxide synthase expression and steroid regulation in the uterus of women with menorrhagia Mol. Hum. Reprod., November 1, 1999; 5(11): 1048 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bani, M. C. Baccari, S. Nistri, F. Calamai, M. Bigazzi, and T. B. Sacchi Relaxin Up-Regulates the Nitric Oxide Biosynthetic Pathway in the Mouse Uterus: Involvement in the Inhibition of Myometrial Contractility Endocrinology, October 1, 1999; 140(10): 4434 - 4441. [Abstract] [Full Text]
|
|
|
|
|
|
A. Manabe, T. Hata, T. Yanagihara, M. Hashimoto, Y. Yamada, S. Irikoma, S. Aoki, S. Masumura, and K. Miyazaki Nitric oxide synthesis is increased after dehydroepiandrosterone sulphate administration in term human pregnancy Hum. Reprod., August 1, 1999; 14(8): 2116 - 2119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Khorram, M. Garthwaite, and R. R. Magness Endometrial and Myometrial Expression of Nitric Oxide Synthase Isoforms in Pre- and Postmenopausal Women J. Clin. Endocrinol. Metab., June 1, 1999; 84(6): 2226 - 2232. [Abstract] [Full Text]
|
|
|
|
|
|
P. Lesny, S.R. Killick, R.L. Tetlow, D.J. Manton, J. Robinson, and S.D. Maguiness Ultrasound evaluation of the uterine zonal anatomy during in-vitro fertilization and embryo transfer Hum. Reprod., June 1, 1999; 14(6): 1593 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yoshiki, T. Kubota, Y. Matsumoto, and T. Aso Expression of inducible nitric oxide synthase in human cultured endometrial stromal cells Mol. Hum. Reprod., April 1, 1999; 5(4): 353 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L. Clifton, J. F. Telfer, A. J. Thompson, I. T. Cameron, T. G. Teoh, S. J. Lye, and J. R. G. Challis Corticotropin-Releasing Hormone and Proopiomelanocortin-Derived Peptides Are Present in Human Myometrium J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3716 - 3721. [Abstract] [Full Text]
|
|
|
|
|
|
F. Facchinetti, A. Gandolfi, M. Longo, and A. Volpe Serum Nitrites Predict the Response to Prostaglandin-Induced Delivery at Term Reproductive Sciences, May 1, 1998; 5(3): 140 - 143. [Abstract] [PDF]
|
|
|
|
|
|
C. Yallampalli, Y.-L. Dong, P. R. Gangula, and L. Fang Role and Regulation of Nitric Oxide in the Uterus During Pregnancy and Parturition Reproductive Sciences, March 1, 1998; 5(2): 58 - 67. [Abstract] [PDF]
|
|
|
|
|
|
M. L. Casey, J. Smith, G. Alsabrook, and P. C. MacDonald Activation of Adenylyl Cyclase in Human Myometrial Smooth Muscle Cells by Neuropeptides J. Clin. Endocrinol. Metab., September 1, 1997; 82(9): 3087 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Clifford, R Rai, H Watson, S Franks, and L Regan Does suppressing luteinising hormone secretion reduce the miscarriage rate? Results of a randomised controlled trial BMJ, June 15, 1996; 312(7045): 1508 - 1511. [Abstract] [Full Text]
|
|