Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Focarelli, R.
Right arrow Articles by Rosati, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Focarelli, R.
Right arrow Articles by Rosati, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 10, No. 10, pp. 2755-2759, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Changes in the sialylglycoconjugate distribution on the human sperm surface during in-vitro capacitation: partial purification of a 20 kDa sialylglycoprotein of capacitated spermatozoa

Riccardo Focarelli, Andrea Giuffrida and Floriana Rosati1

Department of Evolutionary Biology, University of Siena Via Mattioli 4 53100 Siena, Italy

Correspondence: 1 To whom correspondence should be addressed

Changes in the distribution of sialylglycoconjugates on the surface of uncapacitated and in-vitro capacitated human spermatozoa were studied by means of two sialic acid specific lectins [Maackia amurensis agglutinin and Sambucus nigra agglutinin). On the uncapacitated sperm surface, sialylglycoconjugates were found be localized from the post-acrosomal region of the sperm head to the tail middle piece, whereas after invitro capacitation these molecules were only found in a small area of the post-acrosomal region. The surface of capacitated human spermatozoa was also investigated by specifically radiolabelling its terminal sialic acid residues. A 20 kDa glycoprotein, which was partially purified by anion-exchange chromatography, was the main component of the sialylglycoconjugate pattern after in-vitro capacitation.

Key words: capacitation/glycoconjugates/human spermatozoa/lectins/sialic acid


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
C.H. Yeung, F. Perez-Sanchez, S. Schroter, C. Kirchhoff, and T.G. Cooper
Changes of the major sperm maturation-associated epididymal protein HE5 (CD52) on human ejaculated spermatozoa during incubation in capacitation conditions
Mol. Hum. Reprod., July 1, 2001; 7(7): 617 - 624.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. I. Arenas, J. F. Madrid, F. R. Bethencourt, B. Fraile, and R. Paniagua
Identification of N- and O-linked Oligosaccharides in the Human Epididymis
J. Histochem. Cytochem., October 1, 1998; 46(10): 1185 - 1188.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.