Human Reproduction, Vol. 10, No. 10, pp. 2767-2772, 1995
© 1995 European Society of Human Reproduction and Embryology
research-article |
Effects of epidermal growth factor, transforming growth factor 
and androstenedione on follicular growth and aromatization in culture
Laboratories of Human and Animal Reproductive Biology, Institute of Reproduction and Development, Monash University Clayton, Victoria 3168, Australia
Correspondence: 1 To whom correspondence should be addressed
The effects of epidermal growth factor (EGF), transforming growth factor alpha (TGF
) and androstenedione on follicular growth and oestradiol production were studied in cultured mouse preantral follicles. Cultured follicles in the control group showed growth and steroidogenic capacities up to the antral follicle stage day 4 of culture. However, many of these follicles failed to resume their growth during the culture. Increasing concentrations of EGF and TGF decreased the number of follicles reaching the large antral stage at day of culture. All treatments with EGF, TGF and androstenedione caused significant inhibitions in both follicular growth and oestradiol production, regardless of the dose used (1–20 ng/ml). Moreover, no synergistic effect between androstenedione and either of the two growth factors was observed. These results suggest that EGF, TGF and/or androstenedione may play important roles in the modulation of gonadotrophin-controlled ovarian function. The effects observed in this study suggest that the aberration in the regulation of these growth factors may result in the creation and maintenance of certain ovarian disorders, such as the polycystic ovarian disease.
Key words:
EGF/follicle/mouse/PCO/TGF
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