Human Reproduction, Vol. 10, No. 11, pp. 2831-2834, 1995
© 1995 European Society of Human Reproduction and Embryology
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Intracytoplasmic sperm injection in the mouse*
1Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center Sackler School of Medicine, Tel Aviv University Tel Aviv, Israel 2Center for Reproductive Medicine, Brussels Free University Laarbeeklaan 101, 1090 Brussels, Belgium
Correspondence: 3To whom correspondence should be addressed
Intracytoplasmic sperm injection (ICSI) into mouse oocytes involves a very low survival rate. This study was designed to determine why ICSI frequently fails in mice. Metaphase II oocytes were obtained from superovulated 4–6 week old F1 hybrid mice. Spermatozoa were retrieved from the epididymis of 12–14 week old F1 hybrid mice. The spiked microinjection pipette used to inject a spermatozoon into the ooplasm had outer and inner diameters of 10 and 8 µm respectively. The oocytes used in the first part of the study were not activated (group 1). Some oocytes were incubated with calcium ionophore for 5 min (group 2). The injected oocytes were evaluated 6, 20, 48 and 72 h after injection. A total of 143 eggs in each group underwent ICSI. In group 1, sperm heads escaped into the perivitelline space. In all, 63 (47%) of the remaining oocytes were damaged during the injection or had degenerated by the first evaluation. The survival rate was 53%, but fertilization did not occur. In group 2, 31 oocytes (22%) were damaged during microinjection or soon degenerated. Two oocytes underwent accidental subzonal insemination. Six oocytes were fertilized (4.2%) among the 78% of survivors. After injection, the sperm tail was found in the cytoplasm (27 and 31% in groups 1 and 2 respectively), the perivitelline space (45% in both groups) or protruding through the zona pellucida (28 and 23% respectively). More oocytes degenerated when the tail remained in the cytoplasm, i.e. 78% in group 1 and 36% in group 2.
Key words: assisted fertilization/intracytoplasmic injection/microinsemination
*The study was performed during sabbatical leave of the first author at the Center for Reproductive Medicine, Brussels Free University, Belgium.
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