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Human Reproduction, Vol. 10, No. 11, pp. 2845-2848, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Augmentation by thyroxine of human granulosa cell gonadotrophin-induced steroidogenesis

Anthony N. Wakim1, Sandra L. Polizotto and Dennis R. Burholt

Department of Obstetrics and Gynecology, The Medical College of Pennsylvania and Hahnemann University/Allegheny Campus Pittsburgh, PA 15212, USA

Correspondence: 1To whom correspondence should be addressed

The objective of this study was to investigate the influence of thyroid hormone on gonadotrophin-induced oestradiol and progesterone secretion by human granulosa cells maintained in vitro. Granulosa cells were obtained by aspiration of pre-ovulatory follicles from women undergoing assisted reproductive technology. Ovulation induction was performed with gonadotrophin-releasing hormone agonist, human menopausal gonadotrophin and human chorionic gonadotrophin. Granulosa cells were maintained in vitro in a defined medium with added insulin. Between 48 and 72 h after the initiation of cell culture, oestradiol and progesterone secretion into the medium was determined for granulosa cells growing in serum-free medium with follicle-stimulating hormone (FSH)/luteinizing hormone (LH) and in serum-free medium with FSH/LH and thyroxine added in a concentration range of 10–10–10–7 M. All concentrations of thyroxine used produced a statistically significant increase in oestradiol (range 1.18–1.37 times the amount with FSH/LH alone) and progesterone (range 1.29–1.51 times the amount with FSH/LH alone) secretion.

Key words: human granulosa cells/oestradiol secretion/progesterone secretion/thyroid hormone


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