Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Gil, T.
Right arrow Articles by Herruzo, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gil, T.
Right arrow Articles by Herruzo, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 10, No. 11, pp. 2923-2927, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

CD4+ cells in human ejaculates

Teresa Gil1, Jose A. Castilla2,4, M.Luisa Hortas1, Jorge Molina1, Maximino Redondo3, Francisco Samaniego1, Federico Garrido1, Francisco Vergara2 and Alfonso Herruzo2

1Servicio Analisis Clinicos 2Unidad de Reproducción Humana del Departamento de Obstetricia y Ginecología, Hospital ‘Virgen de las Nieves’ E-18014 Granada 3Area de Biotecnología, Hospital Costa del Sol Marbella, Spain

Correspondence: 4To whom correspondence should be addressed

Using flow cytometry, we studied the expression of the CD4 antigen within the different cells present in human ejaculate, both in spermatozoa and round cells. In all, 20 samples of semen were obtained from fertile males; in 11 of these, we detected the presence of leukocytes, using the peroxidase test. Swim-up was performed for the analysis of the spermatozoa. From our results it may be concluded that there is no expression of the CD4 antigen on the surface of human spermatozoa or on CD45– ejaculate cells (epithelial and germinal cells). However, we did detect the presence of the CD4 antigen on the surface of the leukocyte cells (CD45+). A better characterization of these CD45+ cells made it apparent that the CD4+ cells of ejaculate are composed of T lymphocytes (helper/inducer T lymphocytes) and monocytes. Thus we may conclude that human spermatozoa do not express the CD4 antigen, the cell surface receptor for human immunodeficiency virus. However, we did detect CD4+ T lymphocytes and CD4+ monocytes in semen.

Key words: flow cytometry/human immunodeficiency virus/semen


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
A. M. Weiler, Q. Li, L. Duan, M. Kaizu, K. L. Weisgrau, T. C. Friedrich, M. R. Reynolds, A. T. Haase, and E. G. Rakasz
Genital Ulcers Facilitate Rapid Viral Entry and Dissemination following Intravaginal Inoculation with Cell-Associated Simian Immunodeficiency Virus SIVmac239
J. Virol., April 15, 2008; 82(8): 4154 - 4158.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
C. Habasque, F. Aubry, B. Jegou, and M. Samson
Study of the HIV-1 receptors CD4, CXCR4, CCR5 and CCR3 in the human and rat testis
Mol. Hum. Reprod., May 1, 2002; 8(5): 419 - 425.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
N. Dejucq and B. Jegou
Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System
Microbiol. Mol. Biol. Rev., June 1, 2001; 65(2): 208 - 231.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
H. Zhang, G. Dornadula, M. Beumont, L. Livornese, B. Van Uitert, K. Henning, and R. J. Pomerantz
Human Immunodeficiency Virus Type 1 in the Semen of Men Receiving Highly Active Antiretroviral Therapy
N. Engl. J. Med., December 17, 1998; 339(25): 1803 - 1809.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.