Skip Navigation

This Article
Right arrow Full Text (PDF )
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mottla, G. L.
Right arrow Articles by Johnson, K. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mottla, G. L.
Right arrow Articles by Johnson, K. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 10, No. 2, pp. 384-391, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryolgoy: Lineage tracing demonstrates that blastomeres of early cleavage-stage human pre-embryos contribute to both trophectoderm and inner cell mass

Gilbert L. Mottla1, Mark R. Adelman3, Jerry L. Hall1, Paul R. Gindoff1, Robert J. Stillman1 and Kurt E. Johnson2

1Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology Washington, DC 20037 2Department of Anatomy, The George Washington University School of Medicine and Health Sciences, Washington, DC 20037 3Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences Bethesda, MD 20895, USA

We injected a fluorescent lineage tracer (Texas Red-lysinedextran) into individual blastomeres of donated human diploid 2- to 8-cell pre-embryos and cultured them to blastocysts. Once pre-embryos reached the expanded blastocyst stage, they were fixed and examined in a scanning confocal microscope to identify the location of fluorescent tracer. In successfully injected pre-embryos that developed to expanded blastocysts, we found that randomly injected blastomeres formed both trophectoderm (TE) and inner cell mass (ICM). More labelled progeny were found in TE than in ICM. Our results show that individual early blastomeres are not yet committed to form either TE or ICM but instead can form both rudiments.

Key words: human pre-embryos/inner cell mass/lineage tracing/trophectoderm


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
F. J. Los, D. Van Opstal, and C. van den Berg
The development of cytogenetically normal, abnormal and mosaic embryos: a theoretical model
Hum. Reprod. Update, January 1, 2004; 10(1): 79 - 94.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. G. Derhaag, E. Coonen, M. Bras, J.M. Bergers Janssen, R. Ignoul-Vanvuchelen, J. P.M. Geraedts, J. L.H. Evers, and J. C.M. Dumoulin
Chromosomally abnormal cells are not selected for the extra-embryonic compartment of the human preimplantation embryo at the blastocyst stage
Hum. Reprod., December 1, 2003; 18(12): 2565 - 2574.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H. Balakier, O. Cabaca, D. Bouman, A. B. Shewchuk, C. Laskin, and J. A. Squire
Spontaneous blastomere fusion after freezing and thawing of early human embryos leads to polyploidy and chromosomal mosaicism
Hum. Reprod., November 1, 2000; 15(11): 2404 - 2410.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Munne, C. Magli, J. Cohen, P. Morton, S. Sadowy, L. Gianaroli, M. Tucker, C. Marquez, D. Sable, A. P. Ferraretti, et al.
Positive outcome after preimplantation diagnosis of aneuploidy in human embryos
Hum. Reprod., September 1, 1999; 14(9): 2191 - 2199.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.