Human Reproduction, Vol. 10, No. 2, pp. 392-395, 1995
© 1995 European Society of Human Reproduction and Embryology
research-article |
Fertilization and early embryology: Embryo quality and pregnancy potential of fresh compared with frozen embryos—is freezing detrimental to high quality embryos?*
Department of Obstetrics and Gynecology, Mount Sinai Medical Center New York, NY 10029, USA
Correspondence: 1To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Bethesda Center for Reproductive Health and Fertility, 619 Oak Street, Cincinnati, OH 45206, USA
To determine the effect of cryopreservation on embryo quality and the pregnancy potential of embryos, donated oocytes from the same donor (n = 24) were randomly allocated, with subsequent transfer to two or more different ovum recipients resulting in at least one fresh and one frozen embryo transfer cycle from the same cohort of oocytes. Endometrial receptivity was controlled in all ovum recipients, and male factor patients were excluded. The number of embryos transferred, mean embryo grade transferred, number of high quality embryos (grade 
2.5, grade 1 being best) transferred and embryo implantation and live birth rates are reported. Significantly more embryos (4.4 ± 1.2 versus 3.3 ± 1.2, P < 0.00003) of higher quality (1.9 ± 0.5 versus 2.1 ± 0.5, P < 0.013) and of a more advanced cell stage (3.0 ± 0.6 versus 2.6 ± 0.7, P < 0.019) were transferred fresh than after cryopreservation respectively. Implantation rates/embryo [19/151 (12.6%) and 9/111 (8.1%)] and live birth rates/transfer [11/42 (26.2%) and 6/45 (13.3%)], from fresh and frozen transfers respectively, were not significantly different despite the larger number of high quality embryos transferred fresh. Embryo cryopreservation adversely affects embryo quality, but does not have detrimental effects on the implantation or pregnancy potential of high quality embryos. Because of the loss of embryos during freeze — thawing during frozen embryo cycles, every effort should be made to attempt a fresh transfer.
Key words: cryopreservation/embryo quality/implantation/invitro fertilization/oocyte donation
2Present address: Instituto Materna-Infantile, Palermo, Italy
3Present address: Department of Obstetrics and Gynecology (SGHO), Travis AFB, CA 94535, USA
4Present address: Department of Obstetrics and Gynecology, New York Medical College, Munger Pavillion, Room 306, Valhalla, NY 10595, USA
*Presented in part at the 41st Annual Meeting of The Society For Gynecologic Investigations, Chicago, Illinois, USA, March 22–25, 1994.
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