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Human Reproduction, Vol. 10, No. 2, pp. 408-414, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryology: Granulosa cell co-culture enhances human embryo development and pregnancy rate following in-vitro fertilization

Melanie R. Freeman1, Christine M. Whitworth and George A. Hill

Center for Assisted Reproduction and Reproductive Endocrinology, Centennial Medical Center 2400 Patterson Street, Suite 319, Nashville, TN 37203, USA

Correspondence: 1To whom correspondence should be addressed

A preliminary study and related clinical trial were performed to evaluate the effects of granulosa-lutein cell co-culture on human embryo development and pregnancy rates for in-vitro fertilization (IVF). In the study, sibling two-pronuclear zygotes were randomly allocated to culture with (co-culture) or without (control) autologous granulosalutein cells. After 24 h, embryos were examined for blastomere number and degree of fragmentation. Co-culture had no effect on the average number of blastomeres per embryo at 24 h; however, fragmentation was significantly decreased in co-cultured embryos (0.7 ± 0.1) compared with controls (1.3 ± 0.2; P < 0.05). In the subsequent clinical trial, all two-pronuclear zygotes were co-cultured for 48 h prior to embryo transfer. The live birth rate per embryo transfer was 43.4% with an implantation rate per embryo of 17.6%. Of the untransferred embryos, 68% developed to the blastocyst stage and were cryopreserved. We conclude that the simple system of autologous granulosa-lutein cell co-culture improves embryo development, implantation and subsequent pregnancy rates for IVF.

Key words: co-culture/embryo culture/granulosa cells/human blastocysts/in-vitro fertilization


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