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Human Reproduction, Vol. 10, No. 3, pp. 505-508, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Physiology: Human tubal fluid: formation and composition during vascular perfusion of the Fallopian tube

C.J. Dickens1, S.D. Maguiness2, M.T. Comer, A. Palmer2, A.J. Rutherford3 and H.J. Leese

1Department of Biology, University of York PO Box No. 373, York YO1 5YW 2The Princess Royal Maternity Hospital Saltshouse Road, Hull HU8 9HE 3Department of Obstetrics and Gynaecology, The General Infirmary at Leeds Leeds LS2 9NS, UK

Correspondence: 1To whom correspondence should be addressed

A vascularly perfused preparation of the Fallopian tube has been developed as a model to study the formation and composition of human tubal fluid. An artery serving the tube was cannulated and perfused at a rate of 0.7 ml/min for 1 h with Medium 199 supplemented with bovine serum albumin, heparin and antibiotics. A cannula was also inserted into the lumen. Light and scanning electron micrographs of control and perfused tubes showed that the epithelial lining was intact after perfusion. Tubal fluid was collected in 13 out of 19 experiments. Fluid could always be collected from patients who were in the follicular phase of their ovarian cycle. The mean rate of appearance was 48 µl/h. The glucose, lactate and pyruvate concentrations in the tubal fluid, as assessed by fluorescence microanalysis, were 0.53, 8.58 and 0.17 mM respectively. There were no correlations between metabolite concentration and the length of perfusion, cannulation time, patient's age or condition. This technique provides a controlled method with which to access and examine human tubal fluid and will allow the physiology of both healthy and diseased tubes to be studied.

Key words: human Fallopian tube/tubal fluid/vascular perfusion


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