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Human Reproduction, Vol. 10, No. 3, pp. 544-550, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Immunology: Distribution of leukocyte subtypes in the sheep ovary after laser drilling

H. Tozawa1,2, M. Brännström2, O. Petrucco2, S. Walker3, H. Chambers4, V. Pascoe5 and R.J. Norman2

2Department of Obstetrics and Gynaecology, University of Adelaide Woodville Road, Woodville, SA 5011 5Department of Histopathology, The Queen Elizabeth Hospital Woodville Road, Woodville, SA 5011 3Turretfield Research Centre, South Australian Research and Development Institute Rosedale, SA 5350 4Department of Histopathology, The Queen Victoria Hospital 160 Fullarton Road, Rose Park, SA 5067, Australia

Correspondence: 1To whom correspondence should be addressed at: Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, The University of Adelaide, The Queen Elizabeth Hospital, Woodville Road, Woodville, SA 5011, Australia

The distribution of leukocyte subtypes in the sheep ovary following laser drilling of the ovarian capsule was examined to understand a possible mechanism by which this treatment promotes ovulation in polycystic ovarian syndrome. Ovaries were removed from sheep at different time-points following laparoscopic laser drilling and immunohisto-chemical detection of leukocyte subtypes, using specific monoclonal antibodies; standard histological staining was performed. Migration of leukocytes into the laser-drilled site was observed as early as 6 h after laser drilling and the total number of leukocytes in the site was found to increase up to the 12th day after surgery. In the earlier period, polymorphonuclear leukocytes were the dominant leukocyte subtypes, while macrophages and lymphocytes were the major cellular components on the 12th day and later. These results show that the tissue changes in the ovary following laser drilling are consistent with a local inflammatory reaction. The prolonged appearance of numerous macrophages following the acute inflammatory phase could lead to the secretion of cytokines and other substances suggested to be important in promoting ovulation. These data indicate that part of the effectiveness of the laser drilling in polycystic ovarian syndrome may be attributable to the secretory products of these leukocytes.

Key words: laser drilling/leukocyte/ovary/ovulation/sheep


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