Human Reproduction, Vol. 10, No. 4, pp. 887-895, 1995
© 1995 European Society of Human Reproduction and Embryology
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Fertilization and early embryolgoy: Effects of granulosa cells and gonadotrophins on meiotic and developmental competence of oocytes in vitro in non-stimulated rhesus monkeys
1Wisconsin Regional Primate Research Center Madison, WI 53715, USA 2Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, WI 53715, USA
Correspondence: 3To whom correspondence should be addressed at: Wisconsin Regional Primate Research Center, 1223 Capitol Court, Madison, WI 53715, USA
Specific aims of this study were to determine effects of granulosa cells and gonadotrophins on the meiotic and developmental competence in vitro of oocytes from non-stimulated rhesus monkeys. Oocytes (368) were obtained from small antral follicles (class 1: 700–999 µm; class 2: 1000–2500 µm) dissected from excised ovaries of 10 follicular and three luteal phase monkeys. Oocytes were cultured in one of four treatment groups: (i) granulosa cells (4x106 live cells/ml) + gonadotrophins (5 µg/ml follicle stimulating hormone and 10 µg/ml luteinizing hormone), (ii) granulosa cells alone, (iii) gonadotrophins alone, or (iv) controls (no granulosa cells or gonadotrophins). Mature oocytes were inseminated and cultured until arrest of embryo development. Meiotic and developmental capacity was greater (P 
0.05) for oocytes from class 2 compared with class 1 follicles and from luteal compared with follicular phase monkeys. Culture of oocytes with gonadotrophins alone during in-vitro maturation improved (P 0.01) activation and cleavage through the 5–8-cell stage (46.5%). Culture with granulosa cells alone during invitro maturation augmented progression of embryos to the morula stage (6.3%). The combination of granulosa cells + gonadotrophins enhanced (P 0.01) nuclear maturation, but had no effects on developmental capacity beyond those of either treatment alone. In conclusion, both granulosa cells and gonadotrophins during in-vitro maturation have stimulatory effects on the meiotic and developmental competence of oocytes from non-stimulated macaques in vitro.
Key words: follicle/gonadotrophins/granulosa cells/in-vitro maturation/macaque oocytes
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