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Human Reproduction, Vol. 10, No. 4, pp. 903-906, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryology: Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen—thawed epididymal spermatozoa

Paul Devroey1, Sherman Silber2, Zsolt Nagy, Jiaen Liu, Herman Tournaye, Hubert Joris, Greta Verheyen and Andre Van Steirteghem

Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University Laarbeeklaan 101, 1090 Brussels, Belgium 2St Luke's Hospital West 224S Woods Mill Road, Suite 730, St Louis, MO 63017, USA

Correspondence: 1To whom correspondence should be addressed

In seven patients who did not become pregnant following microsurgical epididymal sperm aspiration (MESA) and intracytoplasmic sperm injection (ICSI), a subsequent ICSI was performed using previously cryopreserved supernumerary epididymal spermatozoa without re-operating on the husband. During the original MESA procedure a mean sperm concentration of 12.3x106/ml was achieved. The supernumerary spermatozoa were cryopreserved for later use. After thawing frozen epididymal spermatozoa a mean concentration of 1.9x106 spermatozoa/ml was obtained in straws containing a total volume of sperm suspension of 250 µl. From 68 intact oocytes injected with frozen—thawed epididymal spermatozoa, a two pronuclear fertilization rate of 45% and a cleavage rate of 82% were obtained. A total of 17 embryos were replaced in the seven patients, resulting in two ongoing singleton pregnancies and one twin delivery. Six embryos were cryopreserved. In conclusion, it would appear mandatory to cryopreserve supernumerary spermatozoa during a MESA in order to avoid subsequent further scrotal surgery.

Key words: fertilization/frozen-thawed epididymal spermatozoa/intracytoplasmic sperm injection/pregnancy


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