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Human Reproduction, Vol. 10, No. 7, pp. 1694-1701, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Immunology: Diagnostic relevance of abortion-associated human embryonic antigen expressed on the cell surface of tumour promoter-treated Bloom syndrome cells

Yukimasa Shiraishi1,8, Yoshiko Shiraishi1, Dan Yamamoto2, Toshimi Hasegawa2, Wataru Kitamura3, Suzu Miki4, Tadao Tanaka5, Tomohiko Suzuki6 and Hiroaki Soma7

1Laboratories of Human Reproduction and Cell Biology, Department of Anatomy, Kochi Medical School Nankoku-City 783 2Department of Obstetrics and Gynecology, Kochi Prefectural Central Hospital Tokyo 3Kitamura Clinic of Obstetrics and Gynecology, National Ohkura Hospital Tokyo 4Department of Obstetrics and Gynecology, Kochi National Hospital Tokyo 5Department of Obstetrics and Gynecology, National Ohkura Hospital Tokyo 6Department of Biological Chemistry, Faculty of Science, Kochi University Kochi City 780, Koch 7Department of Obstetrics and Gynecology, Saitama Medical School Moroyama-cho 350–04, Saitama, Japan

Correspondence: 8To whom correspondence should be addressed

We detected stable expression of human embryonic antigen associated with spontaneous abortion (HEAA) on the cell surface of a tumour promoter-treated B lymphoblastoid cell line (BS-SHY) originating from Bloom syndrome. We used indirect immunofluorescence and diluted serum from 44 patients who had recurrent spontaneous abortions. With the use of the panning procedure, we separated characteristic cells expressing strong HEAA. The BS-SHY-HEAA cells separated here would be useful for measuring serum antibody (against HEAA) produced by patients with recurrent abortions. It was also noted that aborters who received husbands' leukocyte immunization have lost this antibody, and have delivered successful pregnancies at term. Using HEAA proteins, we conducted Western blotting analysis for the amino acid sequencing (mol. wt 77 kDa). Amino acid sequencing data indicated that HEAA had 87.5% homology to the immunoglobulin (Ig) VHIII region in the framework. Recently, the protective value of high-dose i.v. administration of immunoglobulin in the treatment of recurrent spontaneous abortions has been reported to be similar to that of leukocyte immunization. Therefore, the BS-SHY-HEAA cells appear to provide a valuable tool for rapid serological diagnosis and for evaluating the efficacy of immunotherapy with husbands' leukocytes in patients with recurrent spontaneous abortions.

Key words: amino acid sequencing/Bloom syndrome/human embryonic antigen associated with abortion/immunofluorescence/Western blotting


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