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Human Reproduction, Vol. 10, No. 7, pp. 1757-1760, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Andrology: Undetectable expression of genomic progesterone receptor in human spermatozoa

Jose A. Castilla1,3, Teresa Gil1, Jorge Molina1, Maria Luisa Hortas1, Fernando Rodriguez1, Jorge Torres-Muñoz2, Francisco Vergara2 and Alfonso J. Herruzo2

1Servicio de Análisis Clìnicos, Hospital ‘Virgen de las Nieves’ E-18014 Granada, Spain 2Unidad de Reproducción Humana, Departamento de Obstetricia y Ginecologìa, Centro Matemo-Infantil, Hospital ‘Virgen de las Nieves’ E-18014 Granada, Spain

Correspondence: 3To whom correspondence should be addressed

The expression of genomic progesterone receptor in human ejaculated spermatozoa was investigated. Spermatozoa from 10 fertile donors who exhibited normal semen parameters were analysed. Indirect immunofluorescence and an enzyme immunoassay using monoclonal antibodies against genomic progesterone receptor were used. Different types of spermatozoa were studied: fresh, post-swim-up (migrated), capacitated and post-artificial induction of the acrosome reaction by calcium ionophore A23187. Progestin receptor-rich T47D human breast cancer cells were used as a positive control, and progestin receptor-poor MDA-MB-231 human breast carcinoma cells were used as a negative control. Genomic progesterone receptor was not detected in fresh, migrated, capacitated and post-acrosome reaction induction human spermatozoa and MDA-MB-231 cells by either indirect immunofluorescence or enzyme immunoassay. However, in T47D cells a mean concentration of 1043.2 ± 125.2 fmol genomic progesterone receptor/mg protein was observed by enzyme immunoassay, and indirect immunofluorescence results were positive using both flow cytometry and fluorescence microscopy. These findings suggest that the effect of progesterone on human spermatozoa is not mediated by genomic progesterone receptor.

Key words: genomic progesterone receptor/spermatozoa


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