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Human Reproduction, Vol. 10, No. 7, pp. 1761-1764, 1995
© 1995 European Society of Human Reproduction and Embryology

research-article

Endocrinology: Micromanipulation of mouse gametes with laser microbeam and optical tweezers

M.E. Enginsu5, K. Schütze2, S. Bellanca3, M. Pensis1, R. Campo4, S. Bassil1, J. Donnez1 and S. Gordts1,4,6

1Cliniques Universitaires St-Luc, UCL, Department of Gynecology and Obstetrics, IVF Unit Avenue Hippocrate 10, 1200 Brussels, Belgium; 2Applikatives Laserzentrum Harlaching Sanatoriumplatz 2, D-81545 München, Germany 3Via de Caro 84, 1-95100 Catania Italy 4Medical Center for Fertility Diagnosis IVF-ET, Tiense Vest 168, B-3000 Leuven, Belgium

Correspondence: 6To whom correspondence should be addressed

Micromanipulation of mouse gametes with a commercially available compact laser microbeam system was studied. Both the normal in-vitro fertilization (IVF) group and the laser zona dissection (LZD) group were tested under normal (2X106 motile spermatozoa/ml) and low (500 000 motile spermatozoa/ml) insemination conditions. Subzonal insemination (SUZI) was also tried in a small group of gametes and the results were compared with those of the low insemination groups. Fertilization rates and blastocyst formation rates for the IVF and the LZD-treated groups were respectively 53 and 60% and 60 and 78%, which were not significantly different. However, under low insemination conditions, the results were significantly better in the LZD-treated group (58% fertilization rate and 83% blastocyst formation rate) compared to the results of the IVF group (33 and 48%) (P < 0.05). The SUZI-treated group showed the lowest fertilization rate (18%). No significant difference between the LZD and the IVF group was observed with respect to parthenogenetic activation. LZD has a beneficial effect on fertilization rates in cases of reduced sperm qualit.

Key words: laser/male subfertility/micromanipulation/mouse

5Present address: Hacettepe University, Faculty of Medicine, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, 0611 Ankara, Turkey


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