Human Reproduction, Vol. 10, No. 7, pp. 1897-1906, 1995
© 1995 European Society of Human Reproduction and Embryology
research-article |
Fertilization and development: The stages at which human fertilization arrests: microtubule and chromosome configurations in inseminated oocytes which failed to complete fertilization and development in humans
1Center for Reproductive Health, University of California, Irvine Medical Center 101 The City Drive, Orange, CA 92668 2Department of Zoology, University of Wisconsin 1117 West Johnson Street, Madison, WI 53706, USA 3Department of Obstetrics-Gynecology, University of Wisconsin 1117 West Johnson Street, Madison, WI 53706, USA
Correspondence: 4To whom correspondence should be addressed
The goal of fertilization is the union of one, and only one, sperm nucleus with the female pronucleus within the activated oocyte. For this to occur successfully, several events must transpire, including the incorporation of the entire spermatozoon into the oocyte, the completion of meiotic maturation with the extrusion of the second polar body, the metabolic activation of the previously quiescent oocyte, the decondensation of the sperm nucleus and the maternal chromosomes into the male and female pronuclei respectively, and the cytoplasmic migrations of the pronuclei, which bring them into apposition. Defects in any of these events are lethal to the zygote and might prove to be causes of infertility. In this study, the microtubules and DNA were imaged in inseminated human oocytes that had been discarded as unfertilized. The presence and number of incorporated sperm tails were also documented using a monoclonal antibody specific for the post-translationally modified acetylated-
-tubulin found in the tail, but not the oocyte, microtubules. An analysis of 211 oocytes from failed in-vitro fertilizations from 58 patient couples resulted in the determination of several previously undetectable phases at which fertilization arrests: (i) metaphase II arrest; (ii) arrest after the successful incorporation of the spermatozoon; (iii) arrest after the formation of the sperm aster; (iv) arrest during mitotic cell cycle progression; and (v) arrest during meiotic cell cycle progression. Data on polyspermy and arrested embryonic development are also presented. These results have implications for the diagnosis and treatment of female, as well as male, infertility. They also provide a rationale for the reasonable use of intracytoplasmic sperm injection (ICSI) therapy, although they suggest that cases intractable to this approach will be found. Concerns are raised about the use of seemingly 'unfertilized' but inseminated oocytes for subsequent re-inseminations, ICSI or even research, since the fertilization process might have arrested after sperm penetration. These results demonstrate that the proper union of the parental genomes requires a series of cytoskeletal-mediated events on the oocyte surface and within the oocyte proper, and that failure at any phase results in the arrest of human fertilization.
Key words: arrest/chromosomes/fertilization/human/microtubules
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Terada, H. Hasegawa, T. Ugajin, H. Nabeshima, K. Suzuki, N. Yaegashi, and K. Okamura Independent Spatial and Temporal Functions of Human Sperm Centrosomes After Dispermic Microinjection Into Bovine Oocytes J Androl, September 1, 2009; 30(5): 559 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Swain and T. B. Pool ART failure: oocyte contributions to unsuccessful fertilization Hum. Reprod. Update, September 1, 2008; 14(5): 431 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fernandez-Gonzalez, P. N. Moreira, M. Perez-Crespo, M. Sanchez-Martin, M. A. Ramirez, E. Pericuesta, A. Bilbao, P. Bermejo-Alvarez, J. d. D. Hourcade, F. R. d. Fonseca, et al. Long-Term Effects of Mouse Intracytoplasmic Sperm Injection with DNA-Fragmented Sperm on Health and Behavior of Adult Offspring Biol Reprod, April 1, 2008; 78(4): 761 - 772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ebner, M. Moser, M. Sommergruber, O. Shebl, and G. Tews Incomplete denudation of oocytes prior to ICSI enhances embryo quality and blastocyst development Hum. Reprod., November 1, 2006; 21(11): 2972 - 2977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Comizzoli, D. E. Wildt, and B. S. Pukazhenthi Poor Centrosomal Function of Cat Testicular Spermatozoa Impairs Embryo Development In Vitro after Intracytoplasmic Sperm Injection Biol Reprod, August 1, 2006; 75(2): 252 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pellestor, T. Anahory, and S. Hamamah The chromosomal analysis of human oocytes. An overview of established procedures Hum. Reprod. Update, January 1, 2005; 11(1): 15 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jimenez-Movilla, M. Aviles, M. J. Gomez-Torres, P. J. Fernandez-Colom, M. T. Castells, J. de Juan, A. Romeu, and J. Ballesta Carbohydrate analysis of the zona pellucida and cortical granules of human oocytes by means of ultrastructural cytochemistry Hum. Reprod., August 1, 2004; 19(8): 1842 - 1855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Payne, V. Rawe, J. Ramalho-Santos, C. Simerly, and G. Schatten Preferentially localized dynein and perinuclear dynactin associate with nuclear pore complex proteins to mediate genomic union during mammalian fertilization J. Cell Sci., December 1, 2003; 116(23): 4727 - 4738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Tremoleda, T. van Haeften, T. A. E. Stout, B. Colenbrander, and M. M. Bevers Cytoskeleton and Chromatin Reorganization in Horse Oocytes Following Intracytoplasmic Sperm Injection: Patterns Associated with Normal and Defective Fertilization Biol Reprod, July 1, 2003; 69(1): 186 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V.Y. Rawe, S.B. Olmedo, F.N. Nodar, R. Ponzio, and P. Sutovsky Abnormal assembly of annulate lamellae and nuclear pore complexes coincides with fertilization arrest at the pronuclear stage of human zygotic development Hum. Reprod., March 1, 2003; 18(3): 576 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V.Y. Rawe, Y. Terada, S. Nakamura, C.F. Chillik, S.B. Olmedo, and H.E. Chemes A pathology of the sperm centriole responsible for defective sperm aster formation, syngamy and cleavage Hum. Reprod., September 1, 2002; 17(9): 2344 - 2349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pellestor, B. Andreo, F. Arnal, C. Humeau, and J. Demaille Mechanisms of non-disjunction in human female meiosis: the co-existence of two modes of malsegregation evidenced by the karyotyping of 1397 in-vitro unfertilized oocytes Hum. Reprod., August 1, 2002; 17(8): 2134 - 2145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tesarik, C. Mendoza, and E. Greco Paternal effects acting during the first cell cycle of human preimplantation development after ICSI Hum. Reprod., January 1, 2002; 17(1): 184 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.N. Sallam, A. Farrag, A.F. Agameya, F. Ezzeldin, A. Eid, and A. Sallam The use of a modified hypo-osmotic swelling test for the selection of viable ejaculated and testicular immotile spermatozoa in ICSI Hum. Reprod., February 1, 2001; 16(2): 272 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Balaban, B. Urman, A. Isiklar, C. Alatas, R. Mercan, S. Aksoy, and A. Nuhoglu Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa Hum. Reprod., January 1, 2001; 16(1): 125 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Van Blerkom, P. Davis, and S. Alexander Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence Hum. Reprod., December 1, 2000; 15(12): 2621 - 2633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Menezo and Y. Barak Comparison between day-2 embryos obtained either from ICSI or resulting from short insemination IVF: influence of maternal age Hum. Reprod., August 1, 2000; 15(8): 1776 - 1780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Neuber and R.D. Powers Is the mouse a clinically relevant model for human fertilization failures? Hum. Reprod., January 1, 2000; 15(1): 171 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Han, W.-H. Wang, L. R. Abeydeera, A. L. Petersen, J.-H. Kim, C. Murphy, B. N. Day, and R. S. Prather Pronuclear Location Before the First Cell Division Determines Ploidy of Polyspermic Pig Embryos Biol Reprod, November 1, 1999; 61(5): 1340 - 1346. [Abstract] [Full Text]
|
|
|
|
|
|
L.A.J. Van der Westerlaken, F.M. Helmerhorst, J. Hermans, and N. Naaktgeboren Intracytoplasmic sperm injection: position of the polar body affects pregnancy rate Hum. Reprod., October 1, 1999; 14(10): 2565 - 2569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moomjy, L. T. Colombero, L. L. Veeck, Z. Rosenwaks, and G. D. Palermo Sperm integrity is critical for normal mitotic division and early embryonic development Mol. Hum. Reprod., September 1, 1999; 5(9): 836 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.B. Coulam, C. Goodman, and J.S. Rinehart Colour Doppler indices of follicular blood flow as predictors of pregnancy after in-vitro fertilization and embryo transfer Hum. Reprod., August 1, 1999; 14(8): 1979 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Sutovsky, C Simerly, L Hewitson, and G Schatten Assembly of nuclear pore complexes and annulate lamellae promotes normal pronuclear development in fertilized mammalian oocytes J. Cell Sci., January 10, 1998; 111(19): 2841 - 2854. [Abstract] [PDF]
|
|
|
|
 |
|
 F. Ankel-Simons and J. M. Cummins Misconceptions about mitochondria and mammalian fertilization: Implications for theories on human evolution PNAS, November 26, 1996; 93(24): 13859 - 13863. [Abstract] [Full Text] [PDF]
|
|