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Human Reproduction, Vol. 11, No. 10, pp. 2200-2207, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

Ovary and ovulation

Application of image analysis cytometry in fofficular fluid cells obtained from in-vitro fertilization cycles: relationships to patient's age, oocyte maturity, fertilizability and in-vitro fertilization outcome

Theodoros Agorastos1,4, John Bontis1, Basil Tarlatzis2, Helen Bill2, Theodoros Constantinidis3 and Serge Mantalenakis2

1B' University Clinic of Obstetrics and Gynaecology University of Thessaloniki, Hippokrateion Hospital 2A' University Clinic of Obstetrics and Gynaecology, Aristotle University of Thessaloniki, Hippokrateion Hospital 3Laboratory of Hygiene, Aristotle University of Thessaloniki, Panepistimioupohs 54621 Thessaloniki, Greece

Correspondence: 4To whom correspondence should be addressed at: 87 Mitropoleos Street, 54622 Thessaloniki, Greece

In an in-vitro fertilization (LVF)/embryo transfer pro gramme granulosa cells obtained from 59 individual preovulatory follicles were analysed using multiparameter image analysis cytometry, in an attempt to determine whether their morphometric and DNA-cytometric parameters could prove useful in assessing follicle and oncyte maturity and in predicting fertilizabifity and outcome of these IVF cycles. Almost all morphometric and DNA- cytometric parameters were not correlated with either the patient's age or oocyte maturity, and did not predict oocyte fertilization or occurrence of a clinical pregnancy. The only possible relevant parameter which, despite its inverse correlation to total luteinizing hormone administration, also proved to be inversely correlated to pregnancy outcome (in the seven cases in which a pregnancy occurred), was the percentage of granulosa cell nuclei with increased DNA content (>5c). Finally, if granulosa cells do not reveal euploid polyploidization in spontaneous or induced ovulatory cycles, the detected cells with increased DNA content should be interpreted as aneuploid, i.e. with chromosomal aberrations, and so their presence could also be discussed in connection with the hypothetical risk of prospective neoplastic transformation of the tissue.

Key words: cytometry/follicular fluid cells/image analysis/in vitro fertilization


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