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Human Reproduction, Vol. 11, No. 10, pp. 2223-2229, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryology: Comparison of mouse embryo development in open and microdrop co-culture systems

Richard Sherbahn1, Jonna Frasor, Eva Radwanska, Zvi Binor, Mary Wood-Molo, Michal Hibner, Steve Mack and Richard G. Rawlins

Department of Obstetrics and Gynecology, Rush University Medical Center Chicago, IL, USA

Correspondence: 1To whom correspondence should be addressed

Co-culture with numerous cell lines has been shown to improve in-vitro embryo development. It is usually performed in open culture without an oil overlay, or in relatively large volumes of medium (e.g. 0.5 ml) under oil. We compared the efficacy of open and microdrop co-culture systems using human endometrial and tuba] cell lines and mouse zygotes; Although the mean pH values of the media from the tubal cell cultures (both open and oil-covered) decreased significantly over 5 days of culture, this did not appear to impair embryo development Both co-culture and microdrop culture significantly improved blas-tocyst and hatching blastocyst formation rates. The combination of the two techniques (microdrop and co-culture) demonstrated the highest blastocyst formation and hatching blastocyst formation rates, as well as the highest mean cell numbers in hatching blastocysts. Co-culture in a microdrop is a superior system for mouse embryo culture.

Key words: blastocyst/co-culture/endometnal cell monolayers/microdrop culture/oviductal cell monolayers


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