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Human Reproduction, Vol. 11, No. 12, pp. 2681-2686, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryology: Non-enzymatic formation of formaldehyde in mouse oocyte freezing mixtures

Glenys Karran and Michael Legge1

Department of Biochemistry, University of Otago PO Box 56, Dunedin, New Zealand

Correspondence: 1To whom correspondence should be addressed

Three cryoprotectant solvents, dimethylsulphoxide, 1,2-propanediol and glycerol, were investigated for a non-enzymatic reaction product, formaldehyde. All three cryoprotectants demonstrated a direct relationship between increasing solvent molarity and increasing formaldehyde concentration which was independent of temperature and protein (bovine serum albumin). Medium composition significantly influenced the formaldehyde concentration with HTF ≥ T6 ≥ M16 = M2. The formaldehyde could be effectively removed by reduced glutathione, cysteine and dithiothreitol with cysteine being the most effective scavenging agent A reaction mechanism for this scavenging is proposed. The combination of cysteine and cryoprotectant reduced the zona pellucida ‘hardening’ effect in mouse oocytes.

Key words: cryoprotectants/formaldehyde/non-enzymatic reac-tions/oocytes/thiols


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