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Human Reproduction, Vol. 11, No. 12, pp. 2697-2702, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

Fertilization and early embryology: The protective action of polyvinylpyrrolidone-Percoll during the cryopreservation of mouse 2-cell embryos and its effect on subsequent developmental potential post-thaw in vitro and in vivo

J.L. Titterington1,3 and J. Robinson1,2

1Department of Applied Biology, The University of Hull Hull HU6 7RX 2Hull IVF Unit, Princess Royal Hospital Hull HU8 9HE, UK

Correspondence: 3To whom correspondence should be addressed

The effects of cryopreservation, in media containing (FS3 + ) or omitting (FS3) polyvinylpyrrolidone (PVP) in the form of Percoll (PVP-Percoll), on the survival of 2-cell mouse embryos was studied. Survival and zona pellucida disruption post-thaw, growth (assessed by in-vitro culture until the blastocyst stage) and development in vivo (assessed by implantation and living fetus rates and the birth of live progeny) were all investigated. Initial post-thaw survival showed no statistically significant difference (P ≥ 0.05) between FS3+ (91.1 ± 9.8%) and FS3 (84.5 ± 6.6%). However, there was a statistically significant (P ≤ 0.05) reduction in the incidence of zona damage when the freezing solution contained PVP-Percoll compared to the control (3.6 ± 1.0 and 8.7 ± 0.6% respectively) and a statistically significant (P ≤ 0.05) greater number of embryos developing in vitro to the blastocyst stage (84.8 ±7.1 and 72.3 ± 6.1% respectively). The rates of implantation were not significantly different: 72.2 ± 7.0% for FS3+ and 51.2 ± 30.7% for the non-frozen control group. The percentage of live fetuses was also similar between the experimental and control groups: 27.4 ± 10.6 and 24.3 ± 113% respectively. We conclude that the presence of polymers can protect embryos against cryoinjury and that PVP in the form of PVP-Percoll provides a non-toxic alternative to PVP in its native form, during the cryopreservation of mouse 2-cell embryos.

Key words: cryopreservation/embryos/mouse/Percoll/polyvinyl-pyrrolidone


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H. Joris, E. Van den Abbeel, A.D. Vos, and A. Van Steirteghem
Reduced survival after human embryo biopsy and subsequent cryopreservation
Hum. Reprod., November 1, 1999; 14(11): 2833 - 2837.
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