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Human Reproduction, Vol. 11, No. 4, pp. 752-755, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilization (ICSI)

A. Salzbrunn1, D.M. Benson2, A.F. Holstein2 and W. Schulze1,3

1Department of Andrology University of Hamburg Germany 2Institute of Anatomy, University of Hamburg Germany

Correspondence: 3To whom correspondence should be addressed at: Abteilung ftlr Andrologie, Universitiits-Krankenhaus Eppendorf, Martinistr. 52, 20246 Hamburg, Germany

A new method is described for the enzymatic preparation of testicular tissue to obtain vital spermatozoa for ICSI. The tissue obtained from both testes by biopsy is studied by means of the semi-thin section method as well as being cryo-preserved. If spermatid formation is assured by semi-thin section histology, vital spermatozoa from the cryo-preserved portion of testicular tissue are enzymatically prepared. In this way it is possible to optimize the reproductive medical treatment for those couples in whom an extracorporal fertilization of oocytes by means of testicular spermatozoa is being considered.

Key words: assisted fertilization/human/lCSI/speniiatozoa/testis


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