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Human Reproduction, Vol. 11, No. 4, pp. 830-836, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

Fertilization promoting peptide, a tripeptide similar to thyrotrophin-releasing hormone, stimulates the capacitation and fertilizing ability of human spermatozoa in vitro

C.M. Green1,2, S.M. Cockle3, P.F. Watson2 and L.R. Fraser1,4

1Anatomy and Human Biology Group, King's College London Strand, London WC2R 2LS 2Department of Veterinary Basic Sciences, Royal Veterinary College London 3Department of Biochemistry and Physiology, University of Reading Reading, UK

Correspondence: 4To whom correspondence should be addressed

Recent studies have demonstrated that a prostatic tri-peptide similar in structure to thyrotrophin-releasing hormone (TRH) can stimulated the in-vitro capacitation and fertilizing ability of epididymal mouse spermatozoa. Therefore we have proposed that this tripeptide be referred to as fertilization promoting peptide (FPP). Using chlortetracycline fluorescence analysis and the hamster oocyte penetration test (HOPT), we have obtained evidence that FPP can also promote the capacitation and fertilizing ability of ejaculated human spermatozoa in vitro. FPP (25–200 nM) caused a significant increase in the proportion of B-pattern capacitated cells and a decrease in the proportion of F-pattern uncapacitated cells, with no significant stimulation of acrosomal exocytosis. Comparison of FPP with two structurally similar tripeptides, TRH and pyro-glutamyl phenylalanylprolineamide, at 50 nM revealed that only FPP could significantly promote capacitation. Finally, after a brief exposure to progesterone to induce acrosomal exocytosis in capacitated cells, FPP-treated suspensions penetrated a significantly higher proportion of oocytes than the untreated controls when assessed in the HOPT. The presence of FPP in human seminal plasma at concentrations similar to those used here suggests that, in vivo, FPP may play a positive role in promoting human sperm function.

Key words: capacitation/chlortetracycline/fertilization-promoting peptide/hamster oocyte penetration test/TRH


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