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Human Reproduction, Vol. 11, No. 7, pp. 1457-1462, 1996
© 1996 European Society of Human Reproduction and Embryology

research-article

Andrology: Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry

R. Golan1,5, T.G. Cooper2, Y. Oschry1, F. Oberpenning3, H. Schulze4, L. Shochat1 and L.M. Lewin1

1Department of Clinical Biochemistry and Interdepartmental Equipment Facility, Sackler Medical School Tel Aviv University, Ramat Aviv 69978, Israel 2Institute of Reproductive Medicine D-48149 Muenster, Germany 3Urology Clinic of the University D-48149 Muenster, Germany 4Urology Clinic Marienhospital II D-44627 Herne, Germany

Correspondence: 1To whom correspondence should be addressed

Inasmuch as caput epididymal and even testicular spermatozoa are now being used to generate pregnancies by direct injection into the oocyte, differences in the chromatin of spermatozoa from proximal and distal locations in the epidldymis were studied. Acridine Orange staining was used to investigate chromatin structure in human spermatozoa which had left the testis and were undergoing maturation in the epididymis. Measurement of green and red fluorescence Intensities of human spermatozoa by flow cytometry demonstrated a decrease in binding of Acridine Orange to DNA as the spermatozoa traversed the epididymis. Using spermatozoa from the cauda epididymis as the standard, the percentages of spermatozoa from the efferent duct, proximal corpus epididymis, midcorpus epididymis, distal corpus epididymis, proximal cauda epidldymis and distal cauda epididymis that had matured with regard to chromatin condensation were 28 ± 5, 39 ± 3, 49 ± 5, 64 ± 5, 69 ± 6 and 74 ± 4% respectively. It may be concluded that eggs fertilized by ejaculated spermatozoa receive a more highly condensed form of chromatin than that received by eggs Inseminated with proximal epididymal or testicular spermatozoa.

Key words: Acridine Orange/chromatm condensation/epididymis/flow cytometry/human spermatozoa


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