Human Reproduction, Vol. 11, No. 7, pp. 1487-1491, 1996
© 1996 European Society of Human Reproduction and Embryology
research-article |
Ovary and ovulation: Low temperature storage and grafting of human ovarian tissue
1Division of Obstetrics & Gynaecology, School of Clinical Medicine, Umversity of Leeds Clarendon Wing, LGI, Leeds LS2 9NS 2Service de Gynécologie-Obstetrique I, Centre Hospitalier Regional et Universitaire de Limoges Limoges 87000, France 3Leeds General Infirmaiy Leeds LS2 9NS 4St. James's University Hospital Leeds LS9 7TF, UK
Correspondence: 3To whom correspondence should be addressed
Ovarian tissue storage at low temperatures is a promising new method for protecting young cancer patients from the sterilizing effects of chemotherapy andlor radiotherapy. Tissue can be stored and returned to the body in due course as a thin cortical graft since the primordial follicles are distributed peripherally and are relatively resistant to ischaemla. Slices of tissue donated by healthy patients were trimmed to a uniform size and preserved by slow freezing to liquid nitrogen temperatures for up to 2 months in one of the following cryoprotectants: dimethylsuiphoxide, ethylene glycol, glycerol, propylene glycol. Their viability was assessed by counting follicles in histological sections 18 days after grafting under the kidney capsules of severe combined immunodeficlency (SCID) mice, and the results were expressed as percentages of the numbers in comparable pieces of ungrafted tissue. While only 10% of the total number of follicles was found in the glycerol group compared to controls, significantly higher percentages (44–84%) survived cryopreservation in the other media. The tissues were sterile when frozen and thawed without a cryoprotectant. These results suggest that if comparable results could be achieved by autografting, a patient's fertility should be safeguarded from cytotoxic treatment.
Key words: chemotherapy/cryopreservation/grafting/ovary/radiotherapy
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|
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|
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|
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|
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|
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
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|
|
 |
|
 R Grundy, R G Gosden, M Hewitt, V Larcher, A Leiper, H A Spoudeas, D Walker, and W H B Wallace Personal practice: Fertility preservation for children treated for cancer (1): scientific advances and research dilemmas Arch. Dis. Child., April 1, 2001; 84(4): 355 - 359. [Full Text]
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|
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|
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|
|
H. Newton and P. Illingworth In-vitro growth of murine pre-antral follicles after isolation from cryopreserved ovarian tissue Hum. Reprod., March 1, 2001; 16(3): 423 - 429. [Abstract] [Full Text] [PDF]
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K. Oktay and G. Karlikaya Ovarian Function after Transplantation of Frozen, Banked Autologous Ovarian Tissue N. Engl. J. Med., June 22, 2000; 342(25): 1919 - 1919. [Full Text]
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 |
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