Human Reproduction, Vol 12, 101-106, Copyright © 1997 by Oxford University Press
KT Gunasena, PM Villines, ES Critser and JK Critser
The fertility of mice after autologous transplantation of ovaries, before
or after cryopreservation, was investigated in this study. Female mice were
randomly assigned to either sham-operated (n = 14), ovariectomized (n =
11), fresh (n = 12) or cryopreserved (n = 11) ovarian transplant groups.
Ovaries were cryopreserved in 1.4 M dimethyl sulphoxide (DMSO) by cooling
to -55 degrees C at 0.5 degree C/min (ice nucleation at -7 degrees C),
plunged in liquid nitrogen and then thawed at room temperature. Oestrous
cyclicity was observed 7 days after sham operation or 15 days after fresh
or cryopreserved ovarian transplant. Ovariectomized animals did not
demonstrate oestrous cyclicity but were mated, and no pregnancies resulted.
Live births were recorded from all sham-operated, all fresh transplant, and
8/11 (73%) cryopreserved transplant animals. Overall mean +/- SEM litter
sizes from fresh (4.32 +/- 0.44) and cryopreserved (4.71 +/- 0.57)
transplant groups were smaller (P < 0.05) than those of sham-operated
animals (12.54 +/- 0.44), although the sizes were not significantly
different (P > 0.05) from each other. Animals were mated at least four
times, with four litters of live pups from 4/4 sham-operated, 1/10 fresh
and 1/9 cryopreserved ovarian transplant animals. Litter sizes from pups of
sham-operated and transplant animals were not significantly different from
each other. Following autologous transplantation of mouse ovaries, before
or after cryopreservation, offspring appeared normal, with high rates of
fertility.
ARTICLES
Live births after autologous transplant of cryopreserved mouse ovaries
Cryobiology Research Institute, Methodist Hospital of Indiana, Inc., Indianapolis 46202, USA.
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