Human Reproduction, Vol 12, 2208-2216, Copyright © 1997 by Oxford University Press
T Angelopoulos, L Krey, A McCullough, A Adler and JA Grifo
Although round spermatids have been studied extensively using staining
techniques and electron microscopy, little information is available about
their appearance in living conditions. We describe a method of collecting
and identifying round spermatids from ejaculates and testicular biopsies.
The validity of the selection procedure was confirmed by fluorescence
in-situ hybridization. Based on cell size, morphological characteristics of
nucleus and cytoplasm, and on the nucleus/cytoplasm ratio, we harvested a
population of cells that was 84% haploid. This procedure can be applied to
select spermatids for clinical or research purposes.
ARTICLES
A simple and objective approach to identifying human round spermatids
Department of Obstetrics and Gynecology, New York University Medical Center, NY 10016, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Koscinski, C. Wittemer, J.M. Rigot, M. De Almeida, E. Hermant, and A. Defossez Seminal haploid cell detection by flow cytometry in non-obstructive azoospermia: a good predictive parameter for testicular sperm extraction Hum. Reprod., July 1, 2005; 20(7): 1915 - 1920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Urman, C. Alatas, S. Aksoy, R. Mercan, A. Nuhoglu, A. Mumcu, A. Isiklar, and B. Balaban Transfer at the blastocyst stage of embryos derived from testicular round spermatid injection Hum. Reprod., March 1, 2002; 17(3): 741 - 743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Johnson, C. Staub, W. B. Neaves, and R. Yanagimachi Live human germ cells in the context of their spermatogenic stages Hum. Reprod., August 1, 2001; 16(8): 1575 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Balaban, B. Urman, A. Isiklar, C. Alatas, S. Aksoy, R. Mercan, and A. Nuhoglu Progression to the blastocyst stage of embryos derived from testicular round spermatids Hum. Reprod., June 1, 2000; 15(6): 1377 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sutovsky, J. Ramalho-Santos, R. D. Moreno, R. Oko, L. Hewitson, and G. Schatten On-stage selection of single round spermatids using a vital, mitochondrion-specific fluorescent probe MitoTrackerTM and high resolution differential interference contrast microscopy Hum. Reprod., September 1, 1999; 14(9): 2301 - 2312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cremades, R. Bernabeu, A. Barros, and M. Sousa In-vitro maturation of round spermatids using co-culture on Vero cells Hum. Reprod., May 1, 1999; 14(5): 1287 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gandini, A. Lenzi, F. Lombardo, R. Pacifici, and F. Dondero Immature germ cell separation using a modified discontinuous Percoll gradient technique in human semen Hum. Reprod., April 1, 1999; 14(4): 1022 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Ghazzawi, S. Alhasani, M. Taher, and S. Souso Reproductive capacity of round spermatids compared with mature spermatozoa in a population of azoospermic men Hum. Reprod., March 1, 1999; 14(3): 736 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ziyyat, B. Lassalle, J. Testart, P. Briot, E. Amar, C. Finaz, and A. Lefevre Flow cytometry isolation and reverse transcriptase–polymerase chain reaction characterization of human round spermatids in infertile patients Hum. Reprod., February 1, 1999; 14(2): 379 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lassalle, A. Ziyyat, J. Testart, C. Finaz, and A. Lefevre Flow cytometric method to isolate round spermatids from mouse testis Hum. Reprod., February 1, 1999; 14(2): 388 - 394. [Abstract] [Full Text] [PDF]
|
|