Human Reproduction, Vol 12, 2257-2262, Copyright © 1997 by Oxford University Press
B Rosenbusch, M Schneider and K Sterzik
We have attempted to analyse the chromosome constitution of 77
multipronuclear uncleaved zygotes obtained from our in-vitro fertilization
programme. Complete karyotypes could be established for 51 tripronuclear
cells and eight zygotes with four pronuclei. When compiling the results,
the varying arrangement of the chromosome sets was taken into
consideration. Eighteen tripronuclear zygotes showed three separate haploid
metaphases (distribution pattern n/n/n), 16 cells had one haploid and one
diploid chromosome set (n/2n), and in 15 zygotes the individual sets were
not distinguishable (3n). Two zygotes were in fact tetraploid, the
distribution of metaphases on the slide being n/3n and n/n/2n,
respectively. In tripronuclear zygotes the sex chromosome ratio XXX:XXY:XYY
was 14:16:18, excluding the two tetraploid cells and one zygote with a
23,X/23,X/22,-C or -Y karyotype. Chromosome abnormalities were found in 16
zygotes (31.4%) and included numerical (six cells), structural (four cells)
as well as combinations of numerical and structural alterations (six
cells). Four of the zygotes with four pronuclei (50%) had numerical and/or
structural chromosome aberrations. Excluding two cells with one
uninterpretable metaphase and a 22,-C or -Y karyotype, respectively, the
sex chromosome distribution XXXX:XXXY:XXYY:XYYY was 1:1:2:1 in zygotes with
four pronuclei. Another zygote was found to be pentaploid after fixation.
These results suggest that analysis of multipronuclear zygotes yields
valuable information about cytogenetic abnormalities occurring at the
earliest stage of conception.
ARTICLES
The chromosomal constitution of multipronuclear zygotes resulting from in-vitro fertilization
Department of Gynecology and Obstetrics, University of Ulm, Ulm/Donau, Germany.
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