Human Reproduction, Vol 12, 2617-2622, Copyright © 1997 by Oxford University Press
NJ Wheatcroft, C Salt, A Milford-Ward, ID Cooke and AP Weetman
The development of new techniques for the detection of ovarian antibodies
has challenged early concepts about the rarity of ovarian antibodies in
idiopathic premature ovarian failure (POF), but few attempts have been made
to compare results between assays. We have sought to define the prevalence
of ovarian autoimmunity in a group of 30 idiopathic POF patients compared
to a group of 12 patients with POF plus an associated autoimmune disease
and a group of 38 controls, using an enzyme-linked immunosorbent assay
(ELISA) and indirect immunofluorescence (IFL). Ovarian antibodies were
detected in 27% of idiopathic POF patients by ELISA (not significantly
different compared to POF patients with associated autoimmune disease; P
< 0.0003 compared to controls) but only 7% of these patients were
positive by IFL. In a further, pre-selected group of individuals, all
positive for ovarian antibodies by IFL, 53% had measurable antibodies by
ELISA. Some overlap was therefore demonstrated between the two techniques
but many POF patients had ovarian antibodies detectable by only one method.
Immunoblotting studies revealed that no consistent pattern of binding could
be demonstrated for these patients. These results call into question the
specificity of ovarian antibodies as a marker for autoimmune POF.
ARTICLES
Identification of ovarian antibodies by immunofluorescence, enzyme- linked immunosorbent assay or immunoblotting in premature ovarian failure
Department of Medicine, Northern General Hospital, University of Sheffield, UK.
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