Skip Navigation

This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Afzalpurkar, A.
Right arrow Articles by Gupta, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Afzalpurkar, A.
Right arrow Articles by Gupta, S. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol 12, 2664-2670, Copyright © 1997 by Oxford University Press

ARTICLES

Immunoreactivity and in-vitro effect on human sperm-egg binding of antibodies against peptides corresponding to bonnet monkey zona pellucida-3 glycoprotein

A Afzalpurkar, H Shibahara, A Hasegawa, K Koyama and SK Gupta
Gamete Antigen Laboratory, National Institute of Immunology, New Delhi, India.

The following synthetic peptides were made as immunogens for development of a zona-based contraceptive vaccine: P1, KQPFWLLQGGASRAETSVQPVLVE [amino acids (aa) 23-45 with an additional K at the N-terminus]; P2, FSEEKLVFSLRLMEENC (aa 164-179 with an additional C at the C-terminus and T170 replaced by V); and P3, CSFSKSSNSWFPVEGPADICQCC (aa 300-322). The aa are numbered on the basis of bonnet monkey ZP3 precursor protein. Antibodies against an additional peptide P4, KGDCGTPSHSRRQPHVVSQWSRSA (aa 324-347), significantly inhibits human sperm-oocyte binding. In addition, antibodies against cocktail of peptide-diphtheria toxoid conjugates also significantly inhibit the binding of spermatozoa to zona pellucida in a hemizona assay. These results will further help in the design of an immunocontraceptive vaccine based upon synthetic peptides corresponding to ZP3.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
J. Ringleb, M. Rohleder, and K. Jewgenow
Impact of feline zona pellucida glycoprotein B-derived synthetic peptides on in vitro fertilization of cat oocytes
Reproduction, February 1, 2004; 127(2): 179 - 186.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. K. Govind, A. Hasegawa, K. Koyama, and S. K. Gupta
Delineation of a Conserved B Cell Epitope on Bonnet Monkey (Macaca radiata) and Human Zona Pellucida Glycoprotein-B by Monoclonal Antibodies Demonstrating Inhibition of Sperm-Egg Binding
Biol Reprod, January 1, 2000; 62(1): 67 - 75.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
M. Paterson, M.R. Wilson, Z.A. Jennings, M. van Duin, and R.J. Aitken
Design and evaluation of a ZP3 peptide vaccine in a homologous primate model
Mol. Hum. Reprod., April 1, 1999; 5(4): 342 - 352.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Greenhouse, P. E. Castle, and J. Dean
Antibodies to human ZP3 induce reversible contraception in transgenic mice with `humanized' zonae pellucidae
Hum. Reprod., March 1, 1999; 14(3): 593 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. Hinsch, S. Oehninger, W.-B. Schill, and K.-D. Hinsch
Species specificity of human and murine anti-ZP3 synthetic peptide antisera and use of the antibodies for localization and identification of ZP3 or ZPC domains of functional significance
Hum. Reprod., February 1, 1999; 14(2): 419 - 428.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.