Human Reproduction, Vol 12, 2682-2686, Copyright © 1997 by Oxford University Press
MP Kelly, SL Corson, B Gocial, FR Batzer and JN Gutmann
The use of cryopreserved donor spermatozoa for insemination has become
necessary to decrease the risk from sexually transmitted infectious
diseases. Lower fecundity rates have been reported with this practice.
Efforts have been applied to increase success, including identification of
those sperm characteristics which correlate with increased fecundity. Data
from in-vitro fertilization have revealed sperm morphology, motility and
zona pellucida binding as important sperm parameters. Discontinuous Percoll
gradient preparation yields a high concentration of motile spermatozoa.
Using this preparation for thawed donor spermatozoa, we have identified
post-preparation motility and progression as factors associated with
increased fecundity. Consideration should be given to screening sperm
donors with a freeze- thaw Percoll gradient preparation prior to acceptance
into a donor bank.
ARTICLES
Discontinuous Percoll gradient preparation for donor insemination: determinants for success
Department of Obstetrics and Gynecology, Thomas Jefferson University School of Medicine, Pennsylvania Hospital, Philadelphia, USA.
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