Human Reproduction, Vol 12, 2713-2719, Copyright © 1997 by Oxford University Press
N Sofikitis, Y Yamamoto, T Isoyama and I Miyagawa
We compared the fertilizing capacity of rabbit testicular round spermatids
(i) before appearance of the proacrosomal granules (stage - 1), (ii) with
two to three proacrosomal granules attached to the nucleus (stage -2) and
(iii) with one granule attached to the nucleus (stages -3 to -5).
Testicular samples were minced and observed via confocal scanning laser
microscopy, which allows intracellular observation at high magnification
and the subsequent identification of acrosomal granules and round
spermatids. Nuclei of round spermatids of stage -1 (group A), -2 (group B),
and -3 to -5 (group C) were isolated and injected into mature rabbit
oocytes. At 24 and 72 h post-injection the number of cleaved oocytes and
morulae/blastocysts, respectively, were significantly lower in groups A and
B than group C. These results suggest that the rabbit round spermatid
develops fertilizing potential after the stage at which the coalescence of
the acrosomal granules occurs.
ARTICLES
The early haploid male gamete develops a capacity for fertilization after the coalescence of the proacrosomal granules
Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan.
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