Human Reproduction, Vol 12, 2766-2772, Copyright © 1997 by Oxford University Press
ME Dell'Aquila, YS Cho, P Minoia, V Traina, GM Lacalandra and F Maritato
The aim of this study was to compare the effect of the addition of
follicular fluid (FF) collected from preovulatory follicles with that of
oestrous mare serum (EMS) (acting as the control) to TCM-199 medium on the
in-vitro maturation, fertilization and development of equine
cumulus-enclosed oocytes. Oocytes (<30 mm in diameter) were obtained
from the ovaries of slaughtered mares. After in-vitro maturation in the
presence of the two supplements, their fertilization, cleavage and
developmental potential were compared after conventional in-vitro
fertilization (IVF) or intracytoplasmic sperm injection (ICSI) using
frozen-thawed spermatozoa. Follicular fluid did not increase the maturation
of oocytes to metaphase II stage compared to control. After IVF, there was
no difference in fertilization rates between FF- supplemented oocytes and
controls (7/87, 8.4% of oocytes showing two pronuclei with FF versus 7/116,
6% with EMS; not significant). However, after ICSI, FF-supplemented oocytes
showed significantly increased normal fertilization (32/85, 37.6% of
two-pronuclear oocytes) and developmental potential (15/31, 48% cleavage)
compared to the control oocytes (7/47, 14.9%, P < 0.01; and 2/48, 4%, P
< 0.01, respectively). Overall, ICSI resulted in increased fertilization
rates compared to IVF, regardless of the presence or absence of FF (39/132,
29.5% with ICSI versus 14/203, 6.9%). These results suggest that follicular
fluid supplementation may improve the maturity of equine cumulus-enclosed
oocytes sufficiently for the successful use of ICSI, but not sufficiently
for normal sperm-egg interaction occurring during IVF.
ARTICLES
Effects of follicular fluid supplementation of in-vitro maturation medium on the fertilization and development of equine oocytes after in- vitro fertilization or intracytoplasmic sperm injection
Institute of Reproductive Biology and Obstetrics, Faculty of Veterinary Medicine, University of Bari, Italy.
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