Human Reproduction, Vol 12, 2784-2791, Copyright © 1997 by Oxford University Press
WR Edirisinghe, A Murch, S Junk and JL Yovich
In the present study we have assessed the cytogenetic abnormalities of
unfertilized oocytes from in-vitro fertilization (IVF) and intracytoplasmic
sperm injection (ICSI) programmes during a one year period (July 1995 to
July 1996) with the cytogenetic analysis being carried out in a
double-blind manner. A total of 88 unfertilized ICSI and 85 unfertilized
IVF oocytes were used for the study and of these 51 and 62 oocytes, in each
respective group, were suitable for analysis. The haploidy, diploidy and
aneuploidy rates between ICSI (62.7, 7.8 and 5.9%) and IVF (61.3, 9.7 and
14.5%) groups were similar. A significant inter-patient variation in the
incidence of hypohaploidy was observed within the IVF group. Chromosomal
fragmentation or breakage was observed at a similar rate in both groups of
unfertilized oocytes (23.5 and 14.5% for ICSI and IVF respectively). A
significantly higher proportion of ICSI oocytes contained sperm nuclei
(27/51, 52.9%) than did IVF oocytes (20/62, 32.3%, P < 0.01). The
distribution and state of sperm head chromatin in relation to oocyte
chromosomal complement was studied in both groups. ICSI oocytes contained
decondensed or swollen sperm nuclei in association with haploid oocyte
chromosomes (12/27, 44.4%) or condensed sperm heads in oocytes showing no
chromosomal complements (7/27, 25.9%). In IVF oocytes sperm heads were
either arrested in the condensed state (5/20, 25%), metaphase stage (3/20,
15%) or had undergone premature chromosome condensation (PCC; 6/20, 30%) in
association with haploid oocyte chromosomes. The incidence of PCC was
similar in the two groups. A marked variation in the incidence of total
chromosomal abnormality was observed between patients within both ICSI
(0-75%) and IVF (0-71%) groups indicating a possible similarity in oocyte
quality between the majority of male factor and tubal infertility patients.
The type of sperm used in the two fertilization procedures showed an
increased incidence of chromosomal breakage with ICSI-MESA (microepididymal
sperm aspiration) spermatozoa (4/6, 67%) compared to the ICSI-ejaculated
(6/35, 17.1%; P < 0.05), ICSI-testicular biopsy (2/10, 20%) and
IVF-normospermic (9/62, 14.5%; P < 0.01) spermatozoa. Chromosomal
fragmentation may be associated with the degree of difficulty experienced
at sperm injection, especially with sperm retrieved from the reproductive
tract. Thus chromosomal fragmentation in ICSI may need further
investigation using a larger sample size in order to assess the possible
causative factors.
ARTICLES
Cytogenetic abnormalities of unfertilized oocytes generated from in- vitro fertilization and intracytoplasmic sperm injection: a double- blind study
PIVET Medical Centre, Leederville, Perth, Western Australia.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Delimitreva, R. Zhivkova, E. Isachenko, N. Umland, and P.L. Nayudu Meiotic abnormalities in in vitro-matured marmoset monkey (Callithrix jacchus) oocytes: development of a non-human primate model to investigate causal factors Hum. Reprod., January 1, 2006; 21(1): 240 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gutierrez-Mateo, J. Benet, D. Wells, P. Colls, M.G. Bermudez, J.F. Sanchez-Garcia, J. Egozcue, J. Navarro, and S. Munne Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis Hum. Reprod., December 1, 2004; 19(12): 2859 - 2868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. M. Cruysberg, A. C. Moll, S. M. Imhof, I. Anteby, E. Cohen, E. Anteby, and D. BenEzra Bilateral Sporadic Retinoblastoma in a Child Born After In Vitro Fertilization Arch Ophthalmol, December 1, 2002; 120(12): 1773 - 1774. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Liu, J. R. Trimarchi, and D. L. Keefe Haploidy but Not Parthenogenetic Activation Leads to Increased Incidence of Apoptosis in Mouse Embryos Biol Reprod, January 1, 2002; 66(1): 204 - 210. [Abstract] [Full Text]
|
|
|
|
|
|
I. Anteby, E. Cohen, E. Anteby, and D. BenEzra Ocular Manifestations in Children Born After In Vitro Fertilization Arch Ophthalmol, October 1, 2001; 119(10): 1525 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Neuber and R.D. Powers Is the mouse a clinically relevant model for human fertilization failures? Hum. Reprod., January 1, 2000; 15(1): 171 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.M.M. Kastrop, S.M. Weima, R.J. Van Kooij, and E.R. Te Velde Comparison between intracytoplasmic sperm injection and in-vitro fertilization (IVF) with high insemination concentration after total fertilization failure in a previous IVF attempt Hum. Reprod., January 1, 1999; 14(1): 65 - 69. [Abstract] [Full Text] [PDF]
|
|