Human Reproduction, Vol 12, 328-335, Copyright © 1997 by Oxford University Press
N Desai, D Kinzer, A Loeb and J Goldfarb
This study was designed to provide further information on mouse and human
embryo development in alpha-modified minimum essential medium (alphaMEM).
First, we compared the development and implantation potential of murine
in-vitro fertilized (IVF) embryos cultured in alphaMEM, in the presence and
absence of co-culture cells. No significant difference was observed in
blastocyst rate between alphaMEM alone (76.2%) and alphaMEM plus co-culture
(79.9%). The percentage of hatched blastocysts was, however, higher with
co-culture (47.5 versus 40%, P < 0.01). Transfer of blastocysts to
pseudopregnant foster mothers resulted in similar live birth rates (14.9%
alphaMEM alone versus 19.8% alphaMEM/co-culture). alphaMEM was also
introduced into our clinical IVF programme for culture of human embryos
beyond day 3. Spare human embryos were cultured under oil in microdrops of
alphaMEM supplemented with 10% synthetic serum substitute. Blastocysts were
evaluated for maturity and the presence and organization of the inner cell
mass. A total of 206 embryos from 53 IVF patients underwent extended
culture. The overall blastocyst rate was 45.1%. An inner cell mass was
observed in 76 blastocysts (81.7%). With regard to developmental maturity,
approximately 73% of blastocysts that had been frozen were expanding
(cavity > 50% embryo volume) or fully expanded. These data suggest that
alphaMEM in conjunction with a commercial protein preparation such as
Synthetic Serum Substitute may be a good basal medium for culture of human
embryos to the blastocyst stage.
ARTICLES
Use of Synthetic Serum Substitute and alpha-minimum essential medium for the extended culture of human embryos to the blastocyst stage
Department of Reproductive Biology, Case Western Reserve University, Cleveland, OH 44106, USA.
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