Human Reproduction, Vol 12, 583-585, Copyright © 1997 by Oxford University Press
K Takabatake, H Fujiwara, Y Goto, T Nakayama, T Higuchi, M Maeda and T Mori
To clarify the role of immune cells in the reproductive phenomenon during
early pregnancy, we investigated the effect of splenocytes obtained from
mice in early pregnancy on embryo implantation. We prepared splenocytes
from 5 week old pregnant ICR mice on day 4 (pregnancy day 4 splenocytes;
P4-spl) and day 8 (pregnancy day 8 splenocytes; P8-spl), from 5 week old
pseudopregnant ICR mice on day 4 (pseudopregnancy day 4 splenocytes;
PP4-spl) and from 5 week old di- oestrous virgin mice (di-oestrous
splenocytes; Di-spl). The supernatants (P4-sup, P8-sup, PP4-sup and Di-sup)
derived from the suspensions of P4-spl, P8-spl, PP4-spl and Di-spl
respectively, were used in the control groups. The recipient pseudopregnant
mice (6 weeks of age) were injected i.v. with splenocytes (2 x 10(7) cells)
or supernatants as controls, and embryo transfer was performed on day 2.
Embryo implantation was evaluated 7 days later under laparotomy. The
successful implantation rate per embryo was markedly higher compared with
the control groups in the P4-spl (30.5 +/- 8.55 versus 1.0 +/- 1.37%, n =
200, P < 0.01) and P8-spl (20.1 +/- 10.3 versus 1.17 +/- 1.62%, n = 200,
P < 0.05) groups. On the other hand, a slight but not significant
increase was observed in the PP4-spl (8.33 +/- 8.80% versus 1.5 +/- 1.37%,
n = 200) and Di-spl (9.0 +/- 5.76% versus 2.3 +/- 2.28%, n = 200) groups.
Among the splenocyte administration groups, the implantation rate in the
P4-spl group was significantly higher than in the PP4-spl and Di-spl groups
(P < 0.01). These findings indicate that i.v. administration of
splenocytes can change the murine implantation window. Since the
splenocytes during early pregnancy are most effective on embryo
implantation, peripheral immune cells in early pregnancy may be involved in
embryo implantation.
ARTICLES
Intravenous administration of splenocytes in early pregnancy changes the implantation window in mice
Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Japan.
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