Human Reproduction, Vol 12, 759-768, Copyright © 1997 by Oxford University Press
R Cortvrindt, J Smitz and AC Van Steirteghem
In two consecutive controlled experiments 160 early preantral follicles
were cultured in order to evaluate effects of recombinant follicle
stimulating hormone (r-FSH) on survival, differentiation, oestradiol and
inhibin secretion, cumulus mucification and cumulus-corona-oocyte
detachment by human chorionic gonadotrophin (HCG) stimulation. Nuclear
maturation in oocytes was also assessed following addition of HCG. A
histological analysis of cultured follicles was carried out on semi- thin
sections at various culture stages. Addition of r-FSH was essential for
follicle survival for 16 days: without r-FSH only 11% of the follicles
survived for 12 days (with r-FSH: 79%) and none of these mucified after the
HCG stimulus. r-FSH promoted granulosa cell proliferation and antral-like
cavity formation. Without r-FSH, histology of the cultures demonstrated
degeneration and reduced granulosa cell proliferation; oestradiol and
inhibin production were reduced. This study illustrates the essential role
of FSH in promoting the in-vitro growth of early preantral mouse ovarian
follicles and in maintaining the oocyte under meiotic arrest.
ARTICLES
Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University, Belgium.
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