Human Reproduction, Vol 12, 1006-1012, Copyright © 1997 by Oxford University Press
ST Mortimer, D Schevaert, MA Swan and D Mortimer
For technical reasons sperm head movement is assessed in kinematic
analysis, while flagellar movement is the determining factor of head
movement, not vice versa. It follows then that the development of new
kinematic values to describe the movement of capacitating human spermatozoa
should include the analysis of their flagellar movement. The aim of this
study was to establish quantitative differences between flagellar movement
patterns of hyperactivated and non-hyperactivated spermatozoa which could
then be used in the evaluation of new centroid- based kinematic values.
Spermatozoa were prepared by swim-up from semen into culture medium
supplemented with 30 mg/ml human serum albumin. Sperm movement was recorded
in 50 microm-deep chambers using a 200 Hz video system. Sperm movement was
classified based on flagellar movement, with 24 non-hyperactivated and 26
hyperactivated spermatozoa included in the study. Flagellar analysis was
performed using both a semi-automated analysis system (SIAM FLAG; 30 images
at 200 Hz) and manual methods (100 Hz). Hyperactivated spermatozoa had
significantly larger flagellar beat angles (> or = 87 degrees) and
significantly lower flagellar beat frequencies (< or = 29.4 Hz) than
non- hyperactivated human spermatozoa. In addition, the flagellar wave
amplitude was significantly greater and the bend diameter significantly
smaller for hyperactivated spermatozoa in the proximal region of the
flagellum (up to 20 microm from the head-midpiece junction). The velocity
of the hyperactivated wave was low in this region, although it was
significantly slower than the non-hyperactivated wave in all regions of the
sperm tail.
ARTICLES
Quantitative observations of flagellar motility of capacitating human spermatozoa
Department of Anatomy and Histology and Institute for Biomedical Research, University of Sydney, NSW, Australia.
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