Human Reproduction, Vol 12, 1621-1628, Copyright © 1997 by Oxford University Press
C Gilling-Smith, DS Willis and S Franks
This study analysed the effect of oestradiol on basal and LH-stimulated
production of androstenedione and progesterone by human theca cells in
monolayer culture. Incubations were carried out for either 2 days (seven
experiments) or 4 days (four experiments), in the presence or absence of
luteinizing hormone (LH), oestradiol (10(-9)-10(-6) M) or inhibin. Medium
collected at 48 and 96 h was stored until radioimmunoassay for steroid
content. Theca pooled from small follicles (<10 mm) was used in all but
two experiments; in these, ovaries were obtained from ovulatory women in
the mid-follicular phase of their cycle and theca from small and large
follicles was pooled. Oestradiol inhibited progesterone production in a
dose-dependent manner in all experiments, irrespective of follicle size,
ovulatory status and ovarian morphology, with maximum effect at 10(-6) M.
At this dose, oestradiol had no effect on androstenedione production by
theca from four anovulatory women with polycystic ovaries but produced a
significant augmentation of both basal and LH-stimulated androstenedione
production in theca from five of the seven ovulatory women, with maximal
response in theca from the two pre-ovulatory subjects. During the 48-96 h
period of incubation, oestradiol augmented androstenedione production in
all four experiments and had a greater stimulatory effect than the
physiological dose of inhibin (10 ng/ml). This is the first report of
oestradiol regulating human theca cell steroidogenesis in a dose-dependent
manner.
ARTICLES
Oestradiol feedback stimulation of androgen biosynthesis by human theca cells
Department of Obstetrics and Gynaecology, Imperial College School of Medicine at St Mary's, London, UK.
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