Human Reproduction, Vol 12, 1750-1755, Copyright © 1997 by Oxford University Press
P Xia
The extent to which the morphology of the oocyte at the light microscopy
level is related to the results of intracytoplasmic sperm injection (ICSI)
is controversial. In this study, after cumulus removal, oocytes were graded
into four groups according to the status of the first polar body, size of
the perivitelline space and the presence of cytoplasmic inclusions. Oocyte
data from 65 consecutive patients were reviewed. The results showed that,
for oocytes without cytoplasmic inclusions, the fertilization rate and
embryo development beyond 2-cell stage were significantly lower (P <
0.01) in the oocytes at grade 1-2 (poor) than those in oocytes at grade 3-4
(good). Grade 4 oocytes without inclusions gave the highest proportion
(66.7%) of good embryos with grading 1-2 (grade 1 best; P < 0.01). A
higher proportion of grade 1-2 oocytes (44.7%; P < 0.05) was obtained
from patients older than 35 years. More oocytes containing cytoplasmic
inclusions were seen in patients diagnosed as having female factor
infertility (24.9%; P < 0.01) and older than 35 years (26.5%; P <
0.05) compared to patients with male factor infertility and younger than 35
years. The fertilization rate and embryo development were not associated
with the oestradiol concentration on the day of human chorionic
gonadotrophin administration or the total number of oocytes retrieved. The
results suggest that human oocyte grading based on the triple factors first
polar body, size of perivitelline space and cytoplasmic inclusions is
related significantly to fertilization rate and embryo quality after ICSI.
ARTICLES
Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality
Department of Obstetrics and Gynaecology, McMaster University, Health Sciences Center, Hamilton, Ontario, Canada.
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