Human Reproduction, Vol 12, 1915-1919, Copyright © 1997 by Oxford University Press
J Jeremias, SS David, M Toth and SS Witkin
The 70 kDa heat shock protein (HSP70) is induced in cells exposed to
chemical or physical stress. HSP70 facilitates cell survival by preventing
protein denaturation and incorrect assembly of polypeptides. Induction of
HSP70 messenger RNA (mRNA) synthesis also inhibits transcription of genes
coding for pro-inflammatory cytokines. We analyzed whether HSP70 mRNA was
expressed in a cultured human cervical cell line (HeLa cells) following
exposure to human semen, or in cells obtained from the endocervices of
sexually active women. HeLa cells were co-cultured with a 1:50 dilution of
semen from four men, with purified spermatozoa, or with cell-free seminal
fluid. Endocervical swabs were obtained at mid-cycle from 53 women. HSP70
mRNA was detected in HeLa cells by a reverse transcriptase-polymerase chain
reaction (RT- PCR) and analysis on agarose gels. HSP70 mRNA in cervical
cells was measured by RT-PCR followed by hybridization with an
HSP70-specific internal probe and detection by ELISA. Cervical IgA
antibodies to HSP70 were measured by ELISA. HeLa cell-semen co-culture led
in each case to induction of HSP70 mRNA. Cell-free seminal fluid and
isolated motile spermatozoa also induced HSP70 mRNA when incubated
individually with HeLa cells. HSP70 mRNA was detected in 28 (52.8%) of 53
endocervical cell samples obtained from women at varying times after sexual
intercourse. The percentage of samples expressing HSP70 mRNA was 37.5% at
<10 h, 64.3% at 10 h, 70.0% at 11 h and between 36 and 50% at later
times after semen exposure. Cervical IgA antibodies to HSP70 were also
detected in some women and their occurrence was highly correlated with
HSP70 gene transcription (P < 0.0001). The data demonstrate that
exposure to semen induces HSP70 mRNA in endocervical cells.
ARTICLES
Induction of messenger RNA for the 70 kDa heat shock protein in HeLa cells and the human endocervix following exposure to semen: implications for antisperm antibody production and susceptibility to sexually transmitted infections
Department of Obstetrics and Gynecology, Cornell University Medical College, New York, NY 10021, USA.
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