Human Reproduction, Vol 12, 1985-1992, Copyright © 1997 by Oxford University Press
HS Baillie, AA Pacey, MA Warren, IW Scudamore and CL Barratt
A simple co-culture bioassay system was used to investigate whether or not
the anatomical origin affected the ability of epithelial cells from the
human uterine (Fallopian) tube to 'bind' spermatozoa. This study was also
used to identify some of the factors which may be involved in the
regulation of sperm-epithelial interactions in vitro by comparing different
tissue culture models and assessing the effect of oestradiol concentration.
Epithelial explants harvested from different regions of human uterine tubes
were co-incubated with a known concentration of motile donor spermatozoa.
All results were adjusted to reflect a standard sperm concentration of 5 x
10(6)/ml. More spermatozoa associated per field of isthmic compared to
ampullary epithelium [isthmus 9.5 +/- 0.9, ampulla 7.1 +/- 0.7 (mean +/-
SEM); n = 36, P < 0.05, ANOVA] and cells from post-menopausal patients
had an apparently reduced ability to bind spermatozoa [isthmus 5.5 +/- 2.0,
ampulla 4.3 +/- 1.4 (mean +/- SEM); n = 4]. Neither menstrual cycle stage
nor addition of mid-cycle concentrations of 17beta-oestradiol (750 pmol/l)
affected the number of spermatozoa which bound to epithelium from either
tubal region. In addition, the number of spermatozoa which bound per field
of polarized explants was greater (P < 0.05) than that bound to
dissociated primary and passaged epithelial cell monolayers. This report is
the first to provide evidence suggestive of a role for sperm- epithelial
binding in the formation of an isthmic sperm reservoir in the human uterine
tube. Results also indicate that oestrogen is not involved in the
regulation of these interactions, and that cell polarity is an important
factor for such associations in vitro.
ARTICLES
Greater numbers of human spermatozoa associate with endosalpingeal cells derived from the isthmus compared with those from the ampulla
University Department of Obstetrics and Gynaecology, Jessop Hospital for Women, Sheffield, UK.
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