Human Reproduction, Vol 13, 165-168, Copyright © 1998 by Oxford University Press
Y Hu, W Maxson, D Hoffman, S Ory, S Eager, J Dupre and K Worrilow
Commercially obtained Buffalo rat liver (BRL) cells were grown in monolayer
culture. The effect of BRL cell co-culture with assisted hatching on embryo
development, implantation and pregnancy was investigated in a population of
200 'first-time' in-vitro fertilization (IVF) patients, subdivided into
three groups according to the methods of fertilization [IVF;
intracytoplasmic sperm injection (ICSI); ICSI/IVF]. Assisted hatching was
performed on all embryos chosen for transfer. Following co-culture, the
overall embryo quality, implantation rate and pregnancy rates were not
significantly different from the controls. However, when grouped according
to fertilization method, co-culture was found to have an impact on
pregnancy and implantation rates in the group undergoing conventional IVF.
Using co- culture with assisted hatching, we were able to achieve a 58%
(38/65) clinical pregnancy rate with a 49% (32/65) live birth rate and a
26% (60/235) implantation rate. No changes in the pregnancy and
implantation rates were apparent in ICSI or ICSI/IVF subgroups. This is the
first prospective, randomly controlled study which reports the use of BRL
cell co-culture for human IVF for a large number of patients undergoing IVF
for the first time.
ARTICLES
Co-culture with assisted hatching of human embryos using Buffalo rat liver cells
Northwest Centre for Infertility and Reproductive Endocrinology, Margate, FL 33063, USA.
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