Skip Navigation

This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (126)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Jones, G. M.
Right arrow Articles by Wood, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, G. M.
Right arrow Articles by Wood, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol 13, 169-177, Copyright © 1998 by Oxford University Press

ARTICLES

Evolution of a culture protocol for successful blastocyst development and pregnancy

GM Jones, AO Trounson, DK Gardner, A Kausche, N Lolatgis and C Wood
Centre for Early Human Development, Institute of Reproduction and Development, Monash University, Monash Medical Centre, Australia.

A cell-free culture system was designed for human embryo development to the blastocyst stage by testing a range of culture conditions in a series of protocols. The culture system that was evolved has a brief 1 h exposure to spermatozoa and then culture of the pronucleate zygote for 2 days in IVF-50 medium. Two or three embryos were cultured together in 20 microl microdrops of medium under oil. Embryos were then regrouped and two or three at a similar stage were cultured together in 50 microl microdrops of Gardner's G2 medium under oil from days 3 to 5. Embryos were transferred to fresh G2 medium on day 5 and cultured for a further 1 or 2 days (day 6 or 7). No serum was used in any of the cultures. The embryo transfer medium and G2 medium were supplemented with human serum albumin. The zonae of all blastocysts to be transferred to patients were completely removed enzymatically. Using this protocol, 52% of zygotes developed to blastocysts and 34 out of 35 patients treated received 82 blastocysts and 11 morulae on day 5 or 6. Twenty-one fetal sacs with positive heartbeats (23% implantation rate) were detected in 13 ongoing pregnancies (38% pregnancy rate/transfer or 37%/patient treated). We anticipate that further improvements in embryo development and the selection of viable embryos can be achieved using this simple and effective culture system.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
G. M. Jones, D. S. Cram, B. Song, G. Kokkali, K. Pantos, and A. O. Trounson
Novel strategy with potential to identify developmentally competent IVF blastocysts
Hum. Reprod., August 1, 2008; 23(8): 1748 - 1759.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. J. French, C. A. Adams, L. S. Anderson, J. R. Kitchen, M. R. Hughes, and S. H. Wood
Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts
Stem Cells, February 1, 2008; 26(2): 485 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Kokkali, J. Traeger-Synodinos, C. Vrettou, D. Stavrou, G.M. Jones, D.S. Cram, E. Makrakis, A.O. Trounson, E. Kanavakis, and K. Pantos
Blastocyst biopsy versus cleavage stage biopsy and blastocyst transfer for preimplantation genetic diagnosis of beta-thalassaemia: a pilot study
Hum. Reprod., May 1, 2007; 22(5): 1443 - 1449.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. G. Papanikolaou, E. D'haeseleer, G. Verheyen, H. Van de Velde, M. Camus, A. Van Steirteghem, P. Devroey, and H. Tournaye
Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture. A randomized prospective study
Hum. Reprod., November 1, 2005; 20(11): 3198 - 3203.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Kokkali, C. Vrettou, J. Traeger-Synodinos, G.M. Jones, D.S. Cram, D. Stavrou, A.O. Trounson, E. Kanavakis, and K. Pantos
Birth of a healthy infant following trophectoderm biopsy from blastocysts for PGD of {beta}-thalassaemia major: Case report
Hum. Reprod., July 1, 2005; 20(7): 1855 - 1859.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
P. Schwarzler, H. Zech, M. Auer, K. Pfau, G. Gobel, P. Vanderzwalmen, and N. Zech
Pregnancy outcome after blastocyst transfer as compared to early cleavage stage embryo transfer
Hum. Reprod., September 1, 2004; 19(9): 2097 - 2102.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D.A. Blake, M. Proctor, and N.P. Johnson
The merits of blastocyst versus cleavage stage embryo transfer: a Cochrane review
Hum. Reprod., April 1, 2004; 19(4): 795 - 807.
[Abstract] [PDF]

Home page
 Hum ReprodHome page
E. Coonen, J. G. Derhaag, J. C.M. Dumoulin, L. C.P. van Wissen, M. Bras, M. Janssen, J. L.H. Evers, and J. P.M. Geraedts
Anaphase lagging mainly explains chromosomal mosaicism in human preimplantation embryos
Hum. Reprod., February 1, 2004; 19(2): 316 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Emiliani, A. Delbaere, A.-S. Vannin, J. Biramane, M. Verdoodt, Y. Englert, and F. Devreker
Similar delivery rates in a selected group of patients, for day 2 and day 5 embryos both cultured in sequential medium: a randomized study
Hum. Reprod., October 1, 2003; 18(10): 2145 - 2150.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Lonergan, D. Rizos, A. Gutierrez-Adan, P.M. Moreira, B. Pintado, J. de la Fuente, and M.P. Boland
Temporal Divergence in the Pattern of Messenger RNA Expression in Bovine Embryos Cultured from the Zygote to Blastocyst Stage In Vitro or In Vivo
Biol Reprod, October 1, 2003; 69(4): 1424 - 1431.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. R. Ghassemifar, J. J. Eckert, F. D. Houghton, H. M. Picton, H. J. Leese, and T. P. Fleming
Gene expression regulating epithelial intercellular junction biogenesis during human blastocyst development in vitro
Mol. Hum. Reprod., May 1, 2003; 9(5): 245 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
T. Hardarson, G. Caisander, A. Sjogren, C. Hanson, L. Hamberger, and K. Lundin
A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts
Hum. Reprod., February 1, 2003; 18(2): 399 - 407.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
N.S. Macklon, M.H.E.C. Pieters, M.A. Hassan, P.H.M. Jeucken, M.J.C. Eijkemans, and B.C.J.M. Fauser
A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development
Hum. Reprod., October 1, 2002; 17(10): 2700 - 2705.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I. Van der Auwera, S. Debrock, C. Spiessens, H. Afschrift, E. Bakelants, C. Meuleman, L. Meeuwis, and T. M. D'Hooghe
A prospective randomized study: day 2 versus day 5 embryo transfer
Hum. Reprod., June 1, 2002; 17(6): 1507 - 1512.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G.A. Palmer, J. Traeger-Synodinos, S. Davies, M. Tzetis, C. Vrettou, M. Mastrominas, and E. Kanavakis
Pregnancies following blastocyst stage transfer in PGD cycles at risk for {beta}-thalassaemic haemoglobinopathies
Hum. Reprod., January 1, 2002; 17(1): 25 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Wrenzycki, D. Herrmann, L. Keskintepe, A. Martins Jr, S. Sirisathien, B. Brackett, and H. Niemann
Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos
Hum. Reprod., May 1, 2001; 16(5): 893 - 901.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
F. Devreker, K. Hardy, M. Van den Bergh, A.S. Vannin, S. Emiliani, and Y. Englert
Amino acids promote human blastocyst development in vitro
Hum. Reprod., April 1, 2001; 16(4): 749 - 756.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H. Laverge, P. De Sutter, J. Van der Elst, and M. Dhont
A prospective, randomized study comparing day 2 and day 3 embryo transfer in human IVF
Hum. Reprod., March 1, 2001; 16(3): 476 - 480.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C.-Y. Fong, A. Bongso, H. Sathananthan, J. Ho, and S.-C. Ng
Ultrastructural observations of enzymatically treated human blastocysts: zona-free blastocyst transfer and rescue of blastocysts with hatching difficulties
Hum. Reprod., March 1, 2001; 16(3): 540 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Gerris and E. Van Royen
Avoiding multiple pregnancies in ART: A plea for single embryo transfer
Hum. Reprod., September 1, 2000; 15(9): 1884 - 1888.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Coskun, J. Hollanders, S. Al-Hassan, H. Al-Sufyan, H. Al-Mayman, and K. Jaroudi
Day 5 versus day 3 embryo transfer: a controlled randomized trial
Hum. Reprod., September 1, 2000; 15(9): 1947 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M.C. Magli, G.M. Jones, L. Gras, L. Gianaroli, I. Korman, and A.O. Trounson
Chromosome mosaicism in day 3 aneuploid embryos that develop to morphologically normal blastocysts in vitro
Hum. Reprod., August 1, 2000; 15(8): 1781 - 1786.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Anderiesz, A.-P. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L. Gianaroli, G.M. Jones, and A.O. Trounson
Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro
Hum. Reprod., May 1, 2000; 15(5): 1140 - 1148.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. C.M. Dumoulin, E. Coonen, M. Bras, L. C.P. van Wissen, R. Ignoul-Vanvuchelen, J.M. Bergers-Jansen, J. G. Derhaag, J. P.M. Geraedts, and J. L.H. Evers
Comparison of in-vitro development of embryos originating from either conventional in-vitro fertilization or intracytoplasmic sperm injection
Hum. Reprod., February 1, 2000; 15(2): 402 - 409.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Sjoblom, M. Wikland, and S.A. Robertson
Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro
Hum. Reprod., December 1, 1999; 14(12): 3069 - 3076.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Gerris, D. De Neubourg, K. Mangelschots, E. Van Royen, M. Van de Meerssche, and M. Valkenburg
Prevention of twin pregnancy after in-vitro fertilization or intracytoplasmic sperm injection based on strict embryo criteria: a prospective randomized clinical trial
Hum. Reprod., October 1, 1999; 14(10): 2581 - 2587.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. A. Larson and H. M. Kubisch
The effects of group size on development and interferon-{tau} secretion by in-vitro fertilized and cultured bovine blastocysts
Hum. Reprod., August 1, 1999; 14(8): 2075 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
B. Dale, A. Fiorentino, R. De Stefano, L. Di Matteo, M. l. De Simone, M. Wilding, and F. Zullo
Pregnancies after activated oocyte transfer: a new option for infertility treatment
Hum. Reprod., July 1, 1999; 14(7): 1771 - 1772.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. Gras and A. O. Trounson
Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization: Case report
Hum. Reprod., July 1, 1999; 14(7): 1869 - 1871.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G.M. Jones and A.O. Trounson
The benefits of extended culture
Hum. Reprod., June 1, 1999; 14(6): 1405 - 1408.
[Full Text] [PDF]

Home page
 Hum ReprodHome page
C.-Y. Fong and A. Bongso
Comparison of human blastulation rates and total cell number in sequential culture media with and without co-culture
Hum. Reprod., March 1, 1999; 14(3): 774 - 781.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
B. Behr, T.B. Pool, A.A. Milki, D. Moore, J. Gebhardt, and D. Dasig
Preliminary clinical experience with human blastocyst development in vitro without co-culture
Hum. Reprod., February 1, 1999; 14(2): 454 - 457.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J.M. Lim, B.C. Reggio, R.A. Godke, and W. Hansel
Development of in-vitro-derived bovine embryos cultured in 5% CO2 in air or in 5% O2, 5% CO2 and 90% N2
Hum. Reprod., February 1, 1999; 14(2): 458 - 464.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R.G. Edwards and H. K. Beard
Is the success of human IVF more a matter of genetics and evolution than growing blastocysts?
Hum. Reprod., January 1, 1999; 14(1): 1 - 6.
[Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.