Skip Navigation

This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Tio, S.
Right arrow Articles by de Koning, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tio, S.
Right arrow Articles by de Koning, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 13, No. 10, 2731-2737, October 1998
© 1998 European Society of Human Reproduction and Embryology

Immunoneutralization of follicle stimulating hormone does not affect gonadotrophin surge-inhibiting factor/ attenuating factor bioactivity during the rat ovarian cycle

S. Tio, J.A.M.J. van Dieten and J. de Koning

Leiden Amsterdam Center for Drug Research, Division of Medical Pharmacology, University of Leiden, Sylvius Laboratories, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

Correspondence: To whom correspondence should be addressed

The physiological role of follicle stimulating hormone (FSH) in the regulation of the release of the putative ovarian factor gonadotrophin surge-inhibiting or -attenuating factor (GnSIF/AF) was investigated. Blood FSH concentrations were immunoneutralized in female rats during different days of the ovarian cycle. FSH antiserum was injected in different protocols to neutralize the FSH surge(s) and/or basal FSH concentrations. All animals were killed on the morning of the day of pro-oestrus of the control rats. Hemi-pituitaries were incubated and gonadotrophin-releasing hormone (GnRH) stimulated and basal luteinizing hormone (LH) and FSH releases and pituitary contents were measured. The biological effectiveness of the FSH antiserum was established by monitoring the histology of vaginal smears and inhibin concentrations in trunk-blood. The predominance of small leukocytes in the smears (low oestradiol bioactivity) and the decreased (bioactive) inhibin secretion imply that anti-FSH neutralizes FSH bioactivity. Simultaneously, the results showed that all anti-FSH injections did not affect the biphasic LH response to GnRH in-vitro, which shows an unaltered presence of GnSIF/AF bioactivity in the blood circulation. The results suggest that under physiological conditions GnSIF/AF bioactivity, which keeps the LH responsiveness of the pituitary gland suppressed to the action of GnRH, is not supported by inhibin and not controlled by FSH.

Key words: functional antagonism/GnRH/hyperstimulation/luteinizing hormone/self-priming


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
P. A Fowler and N. Spears
The cultured rodent follicle as a model for investigations of gonadotrophin surge-attenuating factor (GnSAF) production
Reproduction, June 1, 2004; 127(6): 679 - 688.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Tavoulari, S. Frillingos, P. Karatza, I. E. Messinis, and K. Seferiadis
The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor
Hum. Reprod., April 1, 2004; 19(4): 849 - 858.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. de Koning, C.B. Lambalk, F.M. Helmerhorst, and M.N. Helder
Is GnRH self-priming an obligatory feature of the reproductive cycle?
Hum. Reprod., February 1, 2001; 16(2): 209 - 214.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Pappa, K. Seferiadis, T. Fotsis, A. Shevchenko, M. Marselos, O. Tsolas, and I. E.Messinis
Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid
Hum. Reprod., June 1, 1999; 14(6): 1449 - 1456.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.