Human Reproduction, Vol 13, 3338-3344, Copyright © 1998 by Oxford University Press
K Takahashi, H Kurioka, T Ozaki, H Kanasaki, M Kohsaka, K Miyazaki and K Karino
To determine the frequency of a common luteinizing hormone variant in a
Japanese population and to evaluate its significance in infertility, serum
samples were collected from 169 healthy non-pregnant Japanese women, 105
healthy adult Japanese men and 97 female Japanese infertility patients. The
luteinizing hormone variant includes two point mutations in the
beta-subunit gene (Trp8 to Arg8 and Ile15 to Thr15). DNA from blood cells
was studied in 10 healthy women, 10 men and five patients using polymerase
chain reaction and direct sequencing. In immunoassays, results with a
monoclonal antibody recognizing only the wild-type hormone and a polyclonal
antibody recognizing the variant as well were compared as a ratio; ratios
in heterozygotes and in individuals with only wild-type alleles ranged from
0.19 to 0.50 and from 0.56 to 1.21, respectively, and 0.50 was considered a
'cut-off' value for identifying individuals with the variant. For the
larger subject groups, the frequency of the variant was 9.5% in normals.
The mean ratio (0.80 +/- 0.35) in infertility patients was significantly
lower (P < 0.01) than in healthy women (1.09 +/- 0.56), and the variant
occurred more frequently in infertility patients (16.5%) than in healthy
women (8.3%; P < 0.05). The variant was more frequent in patients with
ovulatory disorders (43.8%) than other patients (16.0%; P < 0.05).
ARTICLES
Increased prevalence of luteinizing hormone beta-subunit variant in Japanese infertility patients
Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.
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