Human Reproduction, Vol 13, 3357-3362, Copyright © 1998 by Oxford University Press
JA Hamilton, RK Iles, LK Gunn, CM Wilson, AM Lower, T Chard and JG Grudzinskas
Concentrations of various proteins in uterine flushings have been described
as a direct method for assessment of the secretory activity of the
endometrium. We investigated levels of the endometrial protein known as
placental protein 14 (PP14) in flushings obtained from 271 infertile women.
Under transvaginal ultrasonographic control, 2 ml of 0.154 M sodium
chloride solution were injected into the uterine cavity and re-aspirated,
five times. In contrast to previous studies the recovered volume of each
flushing was not consistent (range: 0.05-2.1 ml); the volume varied
significantly between serial samples obtained from an individual (P = 0.02,
one-way ANOVA), different cycle days (P < 0.0001, one-way ANOVA) and
women with bilaterally blocked versus patent Fallopian tubes (P < 0.05,
Student's t-test). Concentrations of PP14 showed a better correlation with
protein content (r = 0.506, P < 0.0001) than with the recovered volume
(r = 0.087, P = 0.095). We therefore corrected PP14 concentrations for
total protein content as an indicator of the efficiency of the flushing
process. Corrected PP14 concentrations varied significantly relative to
time since the onset of menstruation (P = 0.001, Kruskal Wallis ANOVA) with
higher levels on days 1-8, as previously observed in plasma samples. No
significant difference in PP14 levels was found with different causes of
infertility. This study shows that uterine flushing is not a consistent
process in women with differing physical characteristics and at varying
times throughout the menstrual cycle.
ARTICLES
Concentrations of placental protein 14 in uterine flushings from infertile women: validation of the collection technique and method of expression of results
Academic Department of Obstetrics and Gynaecology, and Fertility Centre, St Bartholomew's and the Royal London School of Medicine and Dentistry, St Bartholomew's Hospital, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Parmar, G. Sachdeva, L. Savardekar, R.R. Katkam, S. Nimbkar-Joshi, S. Gadkar-Sable, V. Salvi, D.D. Manjramkar, P. Meherji, and C.P. Puri Protein repertoire of human uterine fluid duringthe mid-secretory phase of the menstrual cycle Hum. Reprod., February 1, 2008; 23(2): 379 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-H. Baek Aberrant gene expression associated with recurrent pregnancy loss Mol. Hum. Reprod., May 1, 2004; 10(5): 291 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Y. Ng, S. M. Laird, T. C. Li, W. S. B. Yeung, and P. C. Ho Concentrations of endometrial protein PP 14 and CA-125 in uterine flushings performed in natural and stimulated cycles Hum. Reprod., April 1, 2004; 19(4): 905 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Richlin, S. Ramachandran, A. Shanti, A. A. Murphy, and S. Parthasarathy Glycodelin levels in uterine flushings and in plasma of patients with leiomyomas and polyps: implications for implantation Hum. Reprod., October 1, 2002; 17(10): 2742 - 2747. [Abstract] [Full Text] [PDF]
|
|