Human Reproduction, Vol 13, 3414-3417, Copyright © 1998 by Oxford University Press
G Marconi and R Quintana
The Fallopian tube can be damaged by different noxious substances that may
change cellular ultrastructure and function. Alteration of the cell
membrane allows the passage of certain aniline dyes, which can stain the
nucleus. A total of 310 Fallopian tubes from 163 patients who underwent a
surgical or diagnostic laparoscopy during fertility studies was analysed by
salpingoscopy. Cellular nuclei were stained by injection of 20 ml of a 10%
solution of methylene blue in saline solution (NaCl 10%) through the
cervical cannula prior to salpingoscopy. Evaluation of nuclear staining
with methylene blue, adhesions, vascular alterations, and the flattening of
folds in relation to pregnancy outcome was undertaken. Quantification of
salpingoscopic findings was carried out according to a score. Flattening of
folds and vascular alterations showed no difference in the pregnant and
non-pregnant groups. On the other hand, adhesions and nuclear dyeing were
significantly greater in the non-pregnant group (adhesions 13.6 versus
26.8%, P < 0.004, and nuclear dyeing: 25 versus 41.7%, P < 0.009,
pregnant versus non-pregnant). Methylene blue dye is a new tool to evaluate
in vivo cyto-histological tubal damage, and is a useful and simple method
to provide a prognosis of salpingean function.
ARTICLES
Methylene blue dyeing of cellular nuclei during salpingoscopy, a new in- vivo method to evaluate vitality of tubal epithelium
Instituto de Ginecologia y Fertilidad de Buenos Aires (IFER), Argentina.
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